Microbially driven acidity generation in a tailings lake

被引:13
作者
Bernier, L. [1 ]
Warren, L. A. [1 ]
机构
[1] McMaster Univ, Sch Geog & Earth Sci, Hamilton, ON L8S 4K1, Canada
基金
加拿大自然科学与工程研究理事会;
关键词
D O I
10.1111/j.1472-4669.2005.00047.x
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
In situ characterization and geochemical modelling of acid generation in a mine tailings lake (Moose Lake, ON, Canada) over a 2-year period (2001-2002; surficial lake pyrrhotite slurry disposal initiated in 2002) show that bacteria significantly impact acidity behaviour through particle-associated S oxidation and that they do so under conditions that differ from those controlling abiotic pathways. Seasonal epilimnetic pH decreases occurred in both years, decreasing from approximately 3.5 in May to 2.8 by September (2001) or July (2002). Epilimnetic acid generation rates were depth-dependent, with maximal rates observed not at the surface of lake where O-2 concentrations were highest, but rather within a geochemically reactive zone (approximately 1 m thick) of steep, decreasing O-2 gradients and dynamic Fe and S geochemistry in the lower epilimnetic region of the lake. Acid generation occurred dominantly through particle rather than aqueous pathways, but model predictions of acid generation via abiotic pyrrhotite oxidation involving either O-2 or ferric iron (Fe3+) predicted neither the observed rates nor the depths at which maximal rates occurred. In contrast, model predictions based on microbial pathways involving both O-2 and ferric iron (Fe3+) agreed extremely well with both the observed depth profile of H+ generation and the observed rates at any given depth. Imaging showed extensive microbial colonization of epilimnetic-associated pyrrhotite particles commonly with significant biofilm formation. FISH (fluorescence in situ hybridization) probing of the community in both pelagic and particle compartments indicated mixed communities occurred in both, and that Acidithiobacillus spp. accounted for 2-46% of the total community in either compartment. Initiation of pyrrhotite slurry discharge at the lake surface in 2002 was accompanied by a relative increase in the number of particle-associated microbes, as well as a relative proportional decrease of Acidithiobacillus spp. in the total microbial community. Given the widespread occurrence of bacteria across mining environments, the implications of our results extend beyond this specific site and provide new insight into bacterially driven processes contributing to bulk system characteristics which are not currently well constrained.
引用
收藏
页码:115 / 133
页数:19
相关论文
共 70 条
[1]   PHYLOGENETIC IDENTIFICATION AND IN-SITU DETECTION OF INDIVIDUAL MICROBIAL-CELLS WITHOUT CULTIVATION [J].
AMANN, RI ;
LUDWIG, W ;
SCHLEIFER, KH .
MICROBIOLOGICAL REVIEWS, 1995, 59 (01) :143-169
[2]   COLORIMETRIC DETERMINATION OF ELEMENTAL SULFUR IN HYDROCARBONS [J].
BARTLETT, JK ;
SKOOG, DA .
ANALYTICAL CHEMISTRY, 1954, 26 (06) :1008-1011
[3]   A review on pyrrhotite oxidation [J].
Belzile, N ;
Chen, YW ;
Cai, MF ;
Li, YR .
JOURNAL OF GEOCHEMICAL EXPLORATION, 2004, 84 (02) :65-76
[4]   Microbial populations associated with the generation and treatment of acid mine drainage [J].
Benner, SG ;
Gould, WD ;
Blowes, DW .
CHEMICAL GEOLOGY, 2000, 169 (3-4) :435-448
[5]   MINERAL PRODUCTS OF PYRRHOTITE OXIDATION BY THIOBACILLUS-FERROOXIDANS [J].
BHATTI, TM ;
BIGHAM, JM ;
CARLSON, L ;
TUOVINEN, OH .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1993, 59 (06) :1984-1990
[6]   Schwertmannite and the chemical modeling of iron in acid sulfate waters [J].
Bigham, JM ;
Schwertmann, U ;
Traina, SJ ;
Winland, RL ;
Wolf, M .
GEOCHIMICA ET COSMOCHIMICA ACTA, 1996, 60 (12) :2111-2121
[7]   Iron and aluminum hydroxysulfates from acid sulfate waters [J].
Bigham, JM ;
Nordstrom, DK .
SULFATE MINERALS - CRYSTALLOGRAPHY, GEOCHEMISTRY AND ENVIRONMENTAL SIGNIFICANCE, 2000, 40 :351-403
[8]  
BIGLARI M, 2003, C P SUDB 2003 MIN EN
[9]   Geochemical, mineralogical and microbiological characterization of a sulphide-bearing carbonate-rich gold-mine tailings impoundment, Joutel, Quebec [J].
Blowes, DW ;
Jambor, JL ;
Hanton-Fong, CJ ;
Lortie, L ;
Gould, WD .
APPLIED GEOCHEMISTRY, 1998, 13 (06) :687-705
[10]   Characterization of the microbial community of lotic organic aggregates ('river snow') in the Elbe River of Germany by cultivation and molecular methods [J].
Böckelmann, U ;
Manz, W ;
Neu, TR ;
Szewzyk, U .
FEMS MICROBIOLOGY ECOLOGY, 2000, 33 (02) :157-170