A New Assay for Endonuclease/Methyltransferase Activiities Based on Graphene Oxide

被引：86

作者：

Lee, Jieon ^{[2
]}

Kim, Young-Kwan ^{[2
]}

Min, Dal-Hee ^{[1
]}

机构：

[1] Seoul Natl Univ, Dept Chem, Seoul 151747, South Korea

[2] Korea Adv Inst Sci & Technol, Dept Chem, Taejon 305701, South Korea

来源：

ANALYTICAL CHEMISTRY | 2011年 / 83卷 / 23期

基金：

新加坡国家研究基金会;

关键词：

DNA METHYLTRANSFERASES; ENDONUCLEASE ACTIVITY; HOMOLOGOUS RECOMBINATION; S-ADENOSYLMETHIONINE; ENZYME; CANCER; REPAIR; REPLICATION; METHYLATION; INHIBITION;

D O I：

10.1021/ac201298r

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A new endonuclease/methyltransferase activity assay method based on graphene oxide (GO) is developed. Substrate DNA is designed to possess a double-stranded part to serve as a nuclease substrate and a single-stranded part for anchoring the DNA to the GO surface via strong noncovalent binding. Nuclease-mediated DNA hydrolysis induces the recovery of fluorescence intensity of the dye attached to the end of the double-stranded DNA region. This GO-based method allows real-time measurement and quantitative assay for endonuclease/methyltransferase activities in short time.

引用

页码：8906 / 8912

页数：7

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