In vivo models of proliferative vitreoretinopathy

被引：103
作者：
Agrawal, Rajat N. [1 ,3 ]
He, Shikun [1 ,2 ,3 ]
Spee, Christine [1 ,3 ]
Cui, Jing Z. [1 ,4 ]
Ryan, Stephen J. [1 ,3 ]
Hinton, David R. [1 ,2 ,3 ]
机构：
[1] Univ So Calif, Dept Ophthalmol, Los Angeles, CA 90033 USA
[2] Univ So Calif, Dept Pathol, Los Angeles, CA 90033 USA
[3] Univ So Calif, Keck Sch Med, Doheny Eye Inst, Los Angeles, CA 90033 USA
[4] Univ British Columbia, Dept Ophthalmol & Visual Sci, Vancouver, BC V5Z 3N9, Canada
来源：
NATURE PROTOCOLS | 2007年 / 2卷 / 01期
关键词：
D O I：
10.1038/nprot.2007.4
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
We outline current in vitro and in vivo models for experimental proliferative vitreoretinopathy (PVR) and provide a detailed protocol of our standardized in vivo PVR model. PVR is the leading cause of failed surgical procedures for the correction of rhegmatogenous retinal detachment. The pathogenesis of this multifactorial condition is still not completely understood. Experimental models for PVR help us understand the factors that play a role in the pathogenesis of the disease process in a controlled manner and allow for reproducible preclinical assessment of novel therapeutic interventions. We describe a cell injection model in detail that uses homologous retinal pigment epithelial (RPE) cell cultures to induce PVR over a 2-8 week period.
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收藏
页码：67 / 77
页数：11
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