Encapsulation into Stealth Liposomes Enhances the Antitumor Action of Recombinant Cratylia mollis Lectin Expressed in Escherichia coli

被引：10

作者：

da Cunha, Cassia R. A. ^{[1
]}

da Silva, Luis C. N. ^{[1
,2
]}

Almeida, Fabio J. F. ^{[3
]}

Ferraz, Milena S. ^{[3
]}

Varejao, Nathalia ^{[4
]}

de Souza Cartaxo, Marina F. ^{[3
]}

de Miranda, Rita de Cassia M. ^{[5
]}

de Aguiar, Francisco C. A., Jr. ^{[6
]}

da Silva Santos, Noemia P. ^{[6
]}

Coelho, Luana C. B. B. ^{[1
]}

Santos-Magalhaes, Nereide S. ^{[3
]}

dos Santos Correia, Maria T. ^{[1
]}

机构：

[1] Univ Fed Pernambuco, Dept Bioquim, Lab Bioquim Prot, Recife, PE, Brazil

[2] Univ Ceuma, Programa Posgrad Biol Parasitaria, Sao Luis, Brazil

[3] Univ Fed Pernambuco, Lab Imunopatol Keizo Asami, Recife, PE, Brazil

[4] Univ Fed Rio de Janeiro, Inst Bioquim Med, Lab Agregacao Prot & Amiloidoses, Rio De Janeiro, Brazil

[5] Univ Ceuma, Programa Posgrad Meio Ambiente, Sao Luis, Brazil

[6] Univ Fed Pernambuco, Ctr Acad Vitcria, Vitoria De Santo Antao, Brazil

来源：

FRONTIERS IN MICROBIOLOGY | 2016年 / 7卷

关键词：

lectins; heterologous expression; cancer; immunomodulation; pharmaceutical preparations; TUMOR MICROENVIRONMENT; DRUG-DELIVERY; CANCER; INFLAMMATION; EFFICIENCY; CARCINOMA; VESICLES; IMMUNITY; ENZYMES; CELLS;

D O I：

10.3389/fmicb.2016.01355

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

This study evaluated the in vivo antitumor potential of the recombinant lectin from seeds of Cratylia mollis (rCramoll) expressed in Escherichia coil, free or encapsulated in stealth liposomes, using mice transplanted with sarcoma 180. rCramoll loaded stealth liposomes (rCramoll-lipo) were formulated by hydration of the lipid film followed by cycles of freezing and thawing, and about 60% of rCramoll was encapsulated. This novel preparation showed particle size, polydispersity index, and pH suitable for the evaluation of antitumor activity in vivo. Tumor growth inhibition rates were 59% for rCramoll and 75% for rCramoll-lipo. Histopathological analysis of the experimental groups showed that both free and encapsulated lectin caused no changes in the kidneys of animals. Hematological analysis revealed that treatment with rCramoll-lipo significantly increased leukocyte concentration when compared with the untreated and rCramoll group. In conclusion, the encapsulation of rCramoll in stealth liposomes improves its antitumor activity without substantial toxicity; this approach was more successful than the previous results reported for pCramoll loaded into conventional liposomes. At this point, a crucial difference between the antitumor action of free and encapsulated rCramoll was found along with their effects on immune cells. Further investigations are required to elucidate the mechanism(s) of the antitumor effect induced by rCramoll.

引用

页数：11