Determination of fluoride in human whole blood and urine by gas chromatography-mass spectrometry

被引：31

作者：

Kage, Shigetoshi ^{[2
]}

Kudo, Keiko ^{[1
]}

Nishida, Naoki ^{[3
]}

Ikeda, Hideaki ^{[2
]}

Yoshioka, Naofumi ^{[4
]}

Ikeda, Noriaki ^{[1
]}

机构：

[1] Kyushu Univ, Grad Sch Med Sci, Dept Forens Pathol & Sci, Higashi Ku, Fukuoka 8128582, Japan

[2] Fukuoka Prefectural Police Headquarters, Forens Sci Lab, Fukuoka, Japan

[3] Toyama Univ, Dept Legal Med, Grad Sch Med & Pharmaceut Sci, Toyama 930, Japan

[4] Akita Univ, Sch Med, Div Forens Sci, Dept Social Med, Akita 010, Japan

来源：

FORENSIC TOXICOLOGY | 2008年 / 26卷 / 01期

关键词：

fluoride; hydrofluoric acid; gas poisoning; GC-MS; pentafluorobenzyl; whole blood;

D O I：

10.1007/s11419-008-0043-0

中图分类号：

R99 [毒物学（毒理学）];

学科分类号：

100405 ;

摘要：

We developed a simple and sensitive method for determination of fluoride in human whole blood and urine using gas chromatography-mass spectrometry ( GC-MS). Fluoride was alkylated with pentafluorobenzyl bromide in a mixture of acetone and phosphate buffer (pH 6.8). The derivative obtained was analyzed by GC-MS in the positive-ion electron-impact mode. The lower limit of detection for the compound was 0.5 mg/l for both matrices. The calibration curve for fluoride was linear over the concentration range of 1-100 mg/l. The precision and accuracy of the method were evaluated, and relative standard deviation was within 10%. Using this method, levels of fluoride in whole blood and urine were determined in a case of poisoning caused by hydrofluoric acid exposure.

引用

页码：23 / 26

页数：4

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