Immunostimulatory activity of polysaccharide-poly(I:C) nanoparticles

被引：20

作者：

Tincer, Gizem ^{[1
]}

Yerlikaya, Seda ^{[1
]}

Yagci, Fuat C. ^{[1
]}

Kahraman, Tamer ^{[1
]}

Atanur, Osman M. ^{[2
]}

Erbatur, Oktay ^{[2
]}

Gursel, Ihsan ^{[1
]}

机构：

[1] Bilkent Univ, Fac Sci, Biotherapeut ODN Res Lab, Dept Mol Biol & Genet, TR-06800 Ankara, Turkey

[2] Cukurova Univ, Fac Sci, Dept Chem, TR-01330 Adana, Turkey

来源：

BIOMATERIALS | 2011年 / 32卷 / 18期

关键词：

Polysaccharides; Poly(I:C); Nanoparticles; Targeted delivery system; TLR; Immunogenicity; GANODERMA-LUCIDUM-POLYSACCHARIDES; TOLL-LIKE RECEPTORS; INNATE IMMUNITY; CPG-OLIGODEOXYNUCLEOTIDES; CATIONIC LIPOSOMES; CELLS; ANTIGEN; DNA; ACTIVATION; OLIGONUCLEOTIDES;

D O I：

10.1016/j.biomaterials.2011.01.028

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Immunostimulatory properties of mushroom derived polysaccharides (PS) as stand-alone agents were tested. Next. PS were nanocomplexed with polyI:C (pIC) to yield stable nanoparticles around 200 nm in size evidenced by atomic force microscopy and dynamic light scattering analyses. PSs were selectively engaged by cells expressing TLR2 and initiated NF kappa B dependent signaling cascade leading to a Th1-biased cytokine/chemokine secretion in addition to bactericidal nitric oxide (NO) production from macrophages. Moreover, cells treated with nanoparticles led to synergistic IL6, production and upregulation of TNF alpha, MIP3 alpha, IFN gamma and IP10 transcript expression. In mice, PS-Ovalbumin-pIC formulation surpassed anti-OVA IgG responses when compared to either PS-OVA or pIC-OVA mediated immunity. Our results revealed that signal transduction initiated both by TLR2 and TLR3 via co-delivery of pIC by PS in nanoparticle depot delivery system is an effective immunization strategy. The present work implicate that the PS and nucleic acid based nanoparticle approach along with protein antigens can be harnessed to prevent infectious diseases. (C) 2011 Elsevier Ltd. All rights reserved.

引用

页码：4275 / 4282

页数：8

共 36 条

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