Dual confinement of high-loading enzymes within metal-organic frameworks for glucose sensor with enhanced cascade biocatalysis

被引:57
作者
Wang, Qiuping [1 ,2 ]
Chen, Min [1 ]
Xiong, Can [1 ]
Zhu, Xiaofei [1 ]
Chen, Cai [1 ]
Zhou, Fangyao [1 ]
Dong, Yun [3 ,4 ]
Wang, Yu [5 ]
Xu, Jie [6 ]
Li, Yimin [1 ]
Liu, Jiandang [3 ,4 ]
Zhang, Hongjun [3 ,4 ]
Ye, Bangjiao [3 ,4 ]
Zhou, Huang [1 ]
Wu, Yuen [1 ,2 ]
机构
[1] Univ Sci & Technol China, Sch Chem & Mat Sci, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Peoples R China
[2] Dalian Natl Lab Clean Energy, Dalian 116023, Peoples R China
[3] Univ Sci & Technol China, State Key Lab Particle Detect & Elect, Hefei 230026, Peoples R China
[4] Univ Sci & Technol China, Hefei Natl Lab Phys Sci Microscale, Hefei 230026, Peoples R China
[5] Univ Sci & Technol China, Dept Chem, CAS Key Lab Soft Matter Chem, Hefei 230026, Anhui, Peoples R China
[6] Soochow Univ, Inst Funct Nano & Soft Mat FUNSOM, Jiangsu Key Lab Carbon Based Funct Mat & Devices, Suzhou 215123, Peoples R China
基金
中国博士后科学基金; 国家重点研发计划;
关键词
Metal organic frameworks; Biocatalysis; Enzyme confinement; Sensor; Glucose detection; Cascade reaction; EMBEDDING ENZYMES; PEROXIDASE-LIKE; ENCAPSULATION; CONVERSION; MOF; FUNCTIONALITY; NANOPARTICLES; SPECTROSCOPY; STABILITY; PROTEINS;
D O I
10.1016/j.bios.2021.113695
中图分类号
Q6 [生物物理学];
学科分类号
071011 ;
摘要
The intrinsically fragile nature and leakage of the enzymes is a major obstacle for the commercial sensor of a continuous glucose monitoring system. Herein, a dual confinement effect is developed in a three dimensional (3D) nanocage-based zeolite imidazole framework (NC-ZIF), during which the high-loading enzymes can be well encapsulated with unusual bioactivity and stability. The shell of NC-ZIF sets the first confinement to prevent enzymes leakage, and the interior nanocage of NC-ZIF provides second confinement to immobilize enzymes and offers a spacious environment to maintain their conformational freedom. Moreover, the mesoporosity of the formed NC-ZIF can be precisely controlled, which can effectively enhance the mass transport. The resulted GOx/ Hemin@NC-ZIF multi-enzymes system could not only realize rapid detection of glucose by colorimetric and electrochemical sensors with high catalytic cascade activity (with an 8.3-fold and 16-fold enhancements in comparison with free enzymes in solution, respectively), but also exhibit long-term stability, excellent selectivity and reusability. More importantly, the based wearable sweatband sensor measurement results showed a high correlation ( 0.84, P < 0.001) with the levels measured by commercial glucometer. The reported dual confinement strategy opens up a window to immobilize enzymes with enhanced catalytic efficiency and stability for clinical-grade noninvasive continuous glucose sensor.
引用
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页数:7
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