High-performance liquid chromatographic separation of rolipram, bupivacaine and omeprazole using a tartardiamide-based stationary phase Influence of flow rate and temperature on the enantioseparation

被引：7

作者：

da Silva, Ivanildo Jose Junior ^{[1
]}

Sartor, Joao Paulo ^{[1
]}

Pires Rosa, Paulo Cesar ^{[1
]}

de Veredas, Vinicius ^{[1
]}

Barreto, Amaro Gomes Junior ^{[1
]}

Santana, Cesar Costapinto ^{[1
]}

机构：

[1] Univ Estadual Campinas, Sch Chem Engn, Dept Biotechnol Proc, BR-13083970 Campinas, SP, Brazil

来源：

JOURNAL OF CHROMATOGRAPHY A | 2007年 / 1162卷 / 01期

基金：

巴西圣保罗研究基金会;

关键词：

liquid chromatography; enantiomeric separation; chiral stationary phases;

D O I：

10.1016/j.chroma.2007.07.008

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Chromatographic separation of the chiral drugs rolipram, bupivacame and omeprazole on a tartardiamide-based stationary phase commercially named Kromasil CHI-TBB is shown in this work. The effect of temperature on the chromatographic separation of the chiral drugs using the Kromasil CHI-TBB stationary phase was determined quantitatively so as to contribute toward the design for the racernic mixtures of the named compound by using chiral columns. A decrease in the retention and selectivity factors was observed, when the column temperature increased. Van't Hoff plots provided the thermodynamic data. The variation of the thermodynamic parameters enthalpy and entropy are clearly negative meaning that the separation is enthalpy controlled. (c) 2007 Elsevier B.V. All rights reserved.

引用

页码：97 / 102

页数：6

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