Residual dipolar couplings as new conformational restraints in isotopically 13C-enriched oligosaccharides

被引：48
|
作者：
Kiddle, GR [1 ]
Homans, SW [1 ]
机构：
[1] Univ St Andrews, Ctr Biomol Sci, FIFE, St Andrews KY16 9ST, Fife, Scotland
来源：
FEBS LETTERS | 1998年 / 436卷 / 01期
关键词：
residual dipolar coupling; oligosaccharide; NMR; Gm(3);
D O I：
10.1016/S0014-5793(98)01112-0
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
We report the measurement of residual dipolar couplings for C-13-enriched NeuBAc alpha 2-3Gal beta 1-4Glc in a dilute liquid-crystalline medium, These couplings provide long-range conformational restraints that hitherto have not been available for oligosaccharides, We utilise these restraints in dynamical simulated annealing calculations, which support current models of the solution behaviour of the trisaccharide. (C) 1998 Federation of European Biochemical Societies.
引用
收藏
页码：128 / 130
页数：3
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