A real-time monitoring system for adherently grown cells

被引：2

作者：

Moscelli, Nicola ^{[1
]}

van den Driesche, Sander ^{[1
]}

Witarski, Wojciech ^{[2
]}

Iuliano, Filippo ^{[2
]}

Vellekoop, Michael J. ^{[1
]}

机构：

[1] Vienna Univ Technol, Inst Sensor & Actuator Syst, Gusshausstr 27-29, A-1040 Vienna, Austria

[2] Slovak Acad Sci, Inst Virol, Bratislava 84245, Slovakia

来源：

EUROSENSORS XXIV CONFERENCE | 2010年 / 5卷

关键词：

Optical detection; CCD sensor; cell monitoring;

D O I：

10.1016/j.proeng.2010.09.154

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present a real-time, compact and low-cost imaging system for cell cultures grown attached in standard disposable multi-well plates. The system comprises a custom made platform to position a standard multi-well plate and a 1/4 '' CCD image sensor aligned, fixed and focused directly at the bottom of the well of interest. The image sensor has a resolution of 640 x 480 pixels. With a lens mounted on top of the CCD sensor we obtained a 3.26 x 2.45 mm(2) field of view, corresponding to a pixel size of about 5 mu m. With our imaging system we have successfully observed adherent epithelial cells (A549): individually, in spheroid shaped cluster, and in a wound healing assay. (C) 2010 Published by Elsevier Ltd.

引用

页码：492 / 495

页数：4