Interaction between human complement and a pectin type polysaccharide fraction, PMII, from the leaves of Plantago major L.

被引:99
作者
Michaelsen, TE
Gilje, A
Samuelsen, AB
Hogåsen, K
Paulsen, BS
机构
[1] Natl Publ Hlth Inst, Dept Vaccinol, N-0403 Oslo, Norway
[2] Univ Oslo, Inst Pharm, Dept Pharmacognosy, N-0316 Oslo, Norway
[3] Univ Oslo, Natl Hosp, Inst Immunol & Rheumatol, N-0316 Oslo, Norway
关键词
D O I
10.1046/j.1365-3083.2000.00801.x
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
The interaction between a pectin type polysaccharide fraction, PMII, isolated from the leaves of Plantago major, and human complement was tested in two different hemolytic complement-fixation tests and in addition by two elisa methods detecting complement-activation products. Sera were used as a complement source of 10 arbitrary human volunteers, individually and as a pool. The complement-fixation tests were designed to measure the concentration of the pectin necessary to inhibit 50% of the hemolysis (ICH50). The elisa tests for complement-activation products were measured in AU/mg using a fully activated serum as a standard. We observed a more than 200-fold difference in ICH50 activity of the PMII pectin in one of the hemolytic tests by varying the individual sera used as complement-source. On the other hand, the elisa complement-activation tests showed no significant variation in activity of the PMII depending on the complement-serum used. The level of antibodies against PMII detected in the complement-sera did not correlate with the ICH50 activity of PMII. The results show that PMII is a potent complement activator with an activity of the same order of magnitude on a weight basis as that of aggregated human immunoglobulin (Ig)G. This activation leads to a complement consumption probably explaining the PMII's effect in the complement-fixation tests. PMII seems to be an activator both on the classical and the alternative pathway of activation. The results might be related to the reported wound-healing effect of the leaves of Plantago major.
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页码:483 / 490
页数:8
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