Production and characterization of interspecific hybrids between Brassica maurorum and crop brassicas

被引:20
作者
Chrungu, B
Verma, N
Mohanty, A
Pradhan, A
Shivanna, KR [1 ]
机构
[1] Univ Delhi, Dept Bot, Delhi 110007, India
[2] Natl Bur Plant Genet Resources, New Delhi 110012, India
[3] Univ Delhi, Dept Genet, Ctr Genet Manipulat Crop Plants, New Delhi 110021, India
关键词
Brassica maurorum; crop brassicas; embryo rescue; wide hybrids; amphidiploids;
D O I
10.1007/s001220051111
中图分类号
S3 [农学(农艺学)];
学科分类号
0901 ;
摘要
Seven interspecific hybrids were produced between Brassica maurorum (female), a wild species resistant to Alternaria blight and white rust, and all the monogenomic (B. campestris, B. nigra and B. oleracea) and digenomic (B. juncea, B. napus and B. oleracea) crop brassicas (male) through embryo rescue. The hybrids were confirmed by means of morphological and cytological studies. All the hybrids were pollen-sterile, Amphidiploids were induced in three of the hybrids: B. maurorum x B. napus. B. maurorum x B. carinata, B. maurorum x B. nigra. The hybrids were also confirmed through DNA analyses for nuclear and organelle genomes using RAPD and RFLP techniques.
引用
收藏
页码:608 / 613
页数:6
相关论文
共 25 条
[1]   NUCLEOTIDE-SEQUENCE OF THE CLUSTERED GENES FOR THE 44-KD CHLOROPHYLL-A APOPROTEIN AND THE 32KD-LIKE PROTEIN OF THE PHOTOSYSTEM-II REACTION CENTER IN THE SPINACH PLASTID CHROMOSOME [J].
ALT, J ;
MORRIS, J ;
WESTHOFF, P ;
HERRMANN, RG .
CURRENT GENETICS, 1984, 8 (08) :597-606
[2]  
Arumugam N, 1996, THEOR APPL GENET, V92, P762, DOI 10.1007/BF00226099
[3]  
ASTROM H, 1995, SEX PLANT REPROD, V8, P61, DOI 10.1007/BF00230890
[4]  
Banga S. S., 1996, OILSEED VEGETABLE BR, P50
[5]   Accessing and exploiting genes of breeding value of distant relatives of crop Brassicas [J].
Chopra, VL ;
Kirti, PB ;
Prakash, S .
GENETICA, 1996, 97 (03) :305-312
[6]  
DELLAPORTA SL, 1984, MOL BIOL PLANTS LAB
[7]   CYTOPLASMIC MALE-STERILITY IN RAPESEED (BRASSICA-NAPUS L) .1. RESTRICTION PATTERNS OF CHLOROPLAST AND MITOCHONDRIAL-DNA [J].
ERICKSON, L ;
GRANT, I ;
BEVERSDORF, W .
THEORETICAL AND APPLIED GENETICS, 1986, 72 (02) :145-150
[8]   INTERGENERIC HYBRIDS BETWEEN ENARTHROCARPUS-LYRATUS, A WILD-SPECIES, AND CROP BRASSICAS [J].
GUNDIMEDA, HR ;
PRAKASH, S ;
SHIVANNA, KR .
THEORETICAL AND APPLIED GENETICS, 1992, 83 (05) :655-662
[9]   In vitro chromosome doubling potential of colchicine, oryzalin, trifluralin, and APM in Brassica napus microspore culture [J].
Hansen, NJP ;
Andersen, SB .
EUPHYTICA, 1996, 88 (02) :159-164
[10]   CROSSABILITY AND CYTOLOGY OF HYBRID PROGENIES IN THE CROSS BETWEEN BRASSICA-CAMPESTRIS AND 3 WILD RELATIVES OF BRASSICA-OLERACEA, B-BOURGEAUI, B-CRETICA AND B-MONTANA [J].
INOMATA, N .
EUPHYTICA, 1993, 69 (1-2) :7-17