Patterned growth of neuronal cells on modified diamond-like carbon substrates

被引：50

作者：

Kelly, Stephen ^{[4
]}

Regan, Edward M. ^{[3
,4
]}

Uney, James B. ^{[4
]}

Dick, Andrew D. ^{[3
]}

McGeehan, Joseph P. ^{[2
]}

Mayer, Eric J. ^{[3
]}

Claeyssens, Frederik ^{[1
]}

机构：

[1] Univ Bristol, Sch Chem, Bristol BS8 1TS, Avon, England

[2] Univ Bristol, Dept Elect & Elect Engn, Bristol BS8 1TH, Avon, England

[3] Univ Bristol, Bristol Eye Hosp, Bristol BS1 2LX, Avon, England

[4] Univ Bristol, Clin Sci S Bristol, Henry Wellcome LINE, Bristol BS8 1TH, Avon, England

[5] Univ Bristol, Sch Chem, Bristol BS8 1TH, Avon, England

来源：

BIOMATERIALS | 2008年 / 29卷 / 17期

基金：

英国工程与自然科学研究理事会; 英国惠康基金;

关键词：

laser ablation; neural cell; carbon; diamond; micropatterning;

D O I：

10.1016/j.biomaterials.2008.03.001

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Diamond-like carbon (DLC) has been explored as a biomaterial with potential use for coating implantable devices and surgical instruments. In this study the interaction of DLC with mammalian neuronal cells has been studied along with its modifications to improve its function as a biomaterial. We describe the use of DLC, oxidised DLC and phosphorus-doped DLC to support the growth and survival of primary central nervous system neurones and neuroblastoma cells. None of these substrates were cytotoxic and primary neurones adhered better to phosphorus-doped DLC than unmodified DLC. This property was used to culture cortical neurones in a predetermined micropattern. This raises the potential of DLC as a biomaterial for central nervous system (CNS) implantation. Furthermore, patterned DLC and phosphorus-doped DLC can direct neuronal growth, generating a powerful tool to study neuronal networks in a spatially distinct way. This study reports the generation of nerve cell patterns via patterned deposition of DLC. (c) 2008 Elsevier Ltd. All rights reserved.

引用

页码：2573 / 2580

页数：8

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