Autosomal Short Tandem Repeat Analysis of Ancient DNA by Coupled Use of Mini- and Conventional STR Kits

被引：10

作者：

Oh, Chang Seok ^{[2
]}

Lee, Sang Jun ^{[2
]}

Park, Jun Bum

Lee, Soong Deok ^{[3
]}

Seo, Seung Bum ^{[3
]}

Kim, Hye Yeon ^{[3
]}

Kim, Jaehyup ^{[4
]}

Kim, Yi-Suk ^{[5
]}

Shin, Dong Hoon ^{[1
,2
]}

机构：

[1] Seoul Natl Univ, Coll Med, Inst Forens Med, Anthropol & Paleopathol Lab, Seoul 110799, South Korea

[2] Seoul Natl Univ, Coll Med, Dept Anat, Seoul 110799, South Korea

[3] Seoul Natl Univ, Coll Med, Dept Forens Med, Seoul 110799, South Korea

[4] Univ Wisconsin, Sch Med & Publ Hlth, Dept Med, Madison, WI 53705 USA

[5] Ewha Womans Univ, Sch Med, Dept Anat, Seoul 158710, South Korea

来源：

JOURNAL OF FORENSIC SCIENCES | 2012年 / 57卷 / 03期

基金：

新加坡国家研究基金会;

关键词：

forensic science; autosomal short tandem repeat genotyping; medieval human sample; AmpFlSTR (R) MiniFiler (TM) kit; AmpFlSTR (R) Identifiler (TM) kit; ancient DNA; Korea; GENETIC-ANALYSIS; HUMAN REMAINS; DEGRADED DNA; AMPFLSTR((R)) MINIFILER(TM); EXTRACTION; CONCORDANCE; BONE; IDENTIFICATION; AMPLICONS; LOCI;

D O I：

10.1111/j.1556-4029.2011.02044.x

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Multiplex autosomal short tandem repeat (STR) genotyping enables researchers to obtain genetic information from ancient human samples. In this study, we tested newly developed AmpFlSTR (R) MiniFiler (TM) kit for autosomal STR analysis of ancient DNA (aDNA), using human femurs (n = 8) collected from medieval Korean tombs. After extracting aDNA from the bones, autosomal STR analyses were repeated for each sample using the AmpFlSTR (R) MiniFiler (TM) and Identifiler (TM) kits. Whereas only 21.87% of larger-sized loci profiles could be obtained with the Identifiler (TM) kit, 75% of the same loci profiles were determined by MiniFiler (TM) kit analysis. This very successful amplification of large-sized STR markers from highly degraded aDNA suggests that the MiniFiler (TM) kit could be a useful complement to conventional STR kit analysis of ancient samples.

引用

页码：820 / 825

页数：6

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