Waveguide evanescent field fluorescence microscopy: high contrast imaging of a domain forming mixed lipid Langmuir-Blodgett monolayer mimicking lung surfactant

被引：9

作者：

Hassanzadeh, Abdollah ^{[1
]}

Mittler, Silvia ^{[1
]}

机构：

[1] Univ Western Ontario, Dept Phys & Astron, London, ON N6A 3K7, Canada

来源：

JOURNAL OF BIOMEDICAL OPTICS | 2011年 / 16卷 / 04期

基金：

加拿大自然科学与工程研究理事会;

关键词：

fluorescence microscopy; evanescent microscopy; Langmuir-Blodgett films; lipids; sensitivity; photobleaching; contrast; PULMONARY SURFACTANT;

D O I：

10.1117/1.3569095

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Waveguide evanescent field fluorescence (WEFF) microscopy is a new development that allows the imaging of contact regions between biological cells and their substratum, as well as imaging of ultrathin films such as monomolecular Langmuir-Blodgett (LB) films. Mixed-lipid monolayer systems mimicking lung surfactant were fabricated on waveguides using the LB technique and imaged by both WEFF and standard wide field epifluorescence microscopy. These two technologies were compared with respect to contrast, photobleaching, and sensitivity. It was found that WEFF microscopy produced images with a much higher contrast, lower photobleaching, and higher sensitivity. In addition, fine structures in the lipidic domains were observed for the first time. (C) 2011 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.3569095]

引用

页数：4