Isolation of the stable strain Labrys sp BK-8 for L(+)-tartaric acid production

被引：14

作者：

Bao, Wenna ^{[1
,2
]}

Pan, Haifeng ^{[1
,2
]}

Zhang, Zhenhong ^{[1
]}

Cheng, Yongqing ^{[1
]}

Xie, Zhipeng ^{[1
,2
]}

Zhang, Jianguo ^{[1
,2
]}

机构：

[1] Zhejiang Univ, Inst Biochem, Coll Life Sci, Hangzhou 310058, Zhejiang, Peoples R China

[2] Hangzhou Bioking Biochem Engn Co Ltd, Hangzhou 311106, Zhejiang, Peoples R China

来源：

JOURNAL OF BIOSCIENCE AND BIOENGINEERING | 2015年 / 119卷 / 05期

基金：

中国国家自然科学基金;

关键词：

Isolation; cis-Epoxysuccinate hydrolase; Immobilization; L(+)-Tartaric acid; Labrys sp; EPOXIDE HYDROLASE; ESCHERICHIA-COLI; IDENTIFICATION; IMMOBILIZATION; OPTIMIZATION;

D O I：

10.1016/j.jbiosc.2014.10.013

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A novel cis-epoxysuccinate hydrolase (CESH) producing strain of Labrys sp. BK-8 for production of L(+)-tartaric acid was isolated and identified. After optimization, a maximum activity of 3597 +/- 151 U/g was achieved in batch culture in a 10 L fermentor. When Labrys sp. BK-8 was immobilized on kappa-carrageenan, the immobilized cells showed a high conversion rate (>99%), enantioselectivity (EE > 99.5%) and storage stability (>90 d). A conversion rate of 97% was still achieved after 10 repeat batches. The CESH was stable over a broad range of temperatures (up to 45 degrees C) and pH values (4.0-10.0). The Labrys sp. BK-8 isolate provides a new alternative with good stability for the industrial biosynthesis of L(+ )-tartaric add. (C) 2014, The Society for Biotechnology, Japan. All rights reserved.

引用

页码：538 / 542

页数：5

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