Characterization by mass spectroscopy of a 10 kDa c-554 cytochrome from the green sulfur bacterium Chlorobium tepidum

被引：3

作者：

Rémigy, HW

Aivaliotis, M

Ioannidis, N

Jenö, P

Mini, T

Engel, A

Jaquinod, M

Tsiotis, G ^{[1
]}

机构：

[1] Univ Crete, Dept Chem, Div Biochem, Iraklion 71409, Greece

[2] Univ Basel, Bioctr, CH-4056 Basel, Switzerland

[3] Natl Ctr Sci Res Demokritos, Inst Mat Sci, Aghia Paraskevi 15310, Greece

[4] CEA, Dept Biol Mol & Struct, F-38054 Grenoble, France

来源：

PHOTOSYNTHESIS RESEARCH | 2003年 / 78卷 / 02期

关键词：

Chlorobium tepidum; cytochrome; isoelectric focusing; mass spectrometry; peptic cleavage;

D O I：

10.1023/B:PRES.0000004347.34228.2c

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

Preparative isoelectric focusing was used to isolate a type c cytochrome from photosynthetic membranes of the green sulfur bacterium Chlorobium tepidum. The purified protein showed a molecular weight of 10 kDa according to SDS-PAGE and ESI mass spectrometry. The absorption spectrum in the visible range is typical of a cytochrome with peaks at 420, 525.2 and 554.4 nm. Cleavage by either trypsin or endoproteinase lys-C of the isolated cytochrome combined with tandem mass spectrometry and Edman sequencing yielded a sequence perfectly matching parts of the recently sequenced genome of C. tepidum.

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收藏

页码：153 / 160

页数：8

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