Angiostrongylus cantonensis: Identification and characterization of microRNAs in male and female adults

被引：18

作者：

Chen, Mu-Xin ^{[1
,2
]}

Ai, Lin ^{[1
,2
,3
]}

Xu, Min-Jun ^{[2
]}

Zhang, Ren-Li ^{[4
]}

Chen, Shao-Hong ^{[1
]}

Zhang, Yong-Nian ^{[1
]}

Guo, Jian ^{[1
]}

Cai, Yu-Chun ^{[1
]}

Tian, Li-Guang ^{[1
]}

Zhang, Ling-Ling ^{[1
]}

Zhu, Xing-Quan ^{[1
,2
,5
,6
]}

Chen, Jia-Xu ^{[1
]}

机构：

[1] WHO, Natl Inst Parasit Dis, Key Lab Parasite & Vector Biol, Minist Hlth,Chinese Ctr Dis Control & Prevent,Col, Shanghai 200025, Peoples R China

[2] Lanzhou Vet Res Inst, Key Lab Vet Parasitol Gansu Prov, State Key Lab Vet Etiol Biol, Lanzhou 730046, Gansu, Peoples R China

[3] S China Agr Univ, Coll Vet Med, Guangzhou 510642, Guangdong, Peoples R China

[4] Shenzhen Ctr Dis Control & Prevent, Shenzhen 518020, Guangdong, Peoples R China

[5] Heilongjiang Bayi Agr Univ, Coll Anim Sci & Vet Med, Daqing 763379, Heilongjiang, Peoples R China

[6] Yunnan Agr Univ, Coll Anim Sci & Technol, Kunming 650201, Yunnan, Peoples R China

来源：

EXPERIMENTAL PARASITOLOGY | 2011年 / 128卷 / 02期

关键词：

Angiostrongylus cantonensis; Angiostrongyliasis; Eosinophilic meningitis; microRNAs (miRNAs); Solexa deep sequencing; EOSINOPHILIC MENINGITIS; SMALL RNAS; OUTBREAK;

D O I：

10.1016/j.exppara.2011.02.019

中图分类号：

R38 [医学寄生虫学]; Q [生物科学];

学科分类号：

07 ; 0710 ; 09 ; 100103 ;

摘要：

Angiostrongylus cantonensis causes eosinophilic meningitis and eosinophilic pleocytosis in humans and is of significant socio-economic importance globally. microRNAs (miRNAs) are endogenous small non-coding RNAs that play crucial roles in gene expression regulation, cellular function and defense, homeostasis and pathogenesis. They have been identified in a diverse range of organisms. The objective of this study was to determine and characterize miRNAs of female and male adults of A. cantonensis by Solexa deep sequencing. A total of 8861,260 and 10,957,957 high quality reads with 20 and 23 conserved miRNAs were obtained in females and males, respectively. No new miRNA sequence was found. Nucleotide bias analysis showed that uracil was the prominent nucleotide, particularly at positions of 1, 10, 14, 17 and 22, approximately at the beginning, middle and the end of the conserved miRNAs. To our knowledge, this is the first report of miRNA profiles in A. cantonensis, which may represent a new platform for studying regulation of genes and their networks in A. cantonensis. (C) 2011 Elsevier Inc. All rights reserved.

引用

页码：116 / 120

页数：5

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