Cas9-Based Genome Editing in Drosophila

被引：23

作者：

Housden, Benjamin E. ^{[1
]}

Lin, Shuailiang ^{[1
]}

Perrimon, Norbert ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA

[2] Harvard Med Sch, Howard Hughes Med Inst, Boston, MA USA

来源：

USE OF CRISPR/CAS9, ZFNS, AND TALENS IN GENERATING SITE-SPECIFIC GENOME ALTERATIONS | 2014年 / 546卷

关键词：

HOMOLOGOUS RECOMBINATION; KNOCK-IN; CAS9; CRISPR/CAS9; MUTAGENESIS; SPECIFICITY; NUCLEASES; CLEAVAGE; REPAIR;

D O I：

10.1016/B978-0-12-801185-0.00019-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Our ability to modify the Drosophila genome has recently been revolutionized by the development of the CRISPR system. The simplicity and high efficiency of this system allows its widespread use for many different applications, greatly increasing the range of genome modification experiments that can be performed. Here, we first discuss some general design principles for genome engineering experiments in Drosophila and then present detailed protocols for the production of CRISPR reagents and screening strategies to detect successful genome modification events in both tissue culture cells and animals.

引用

页码：415 / 439

页数：25

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