Cas9-Based Genome Editing in Drosophila

被引：23

作者：

Housden, Benjamin E. ^{[1
]}

Lin, Shuailiang ^{[1
]}

Perrimon, Norbert ^{[1
,2
]}

机构：

[1] Harvard Med Sch, Dept Genet, Boston, MA 02115 USA

[2] Harvard Med Sch, Howard Hughes Med Inst, Boston, MA USA

来源：

USE OF CRISPR/CAS9, ZFNS, AND TALENS IN GENERATING SITE-SPECIFIC GENOME ALTERATIONS | 2014年 / 546卷

关键词：

HOMOLOGOUS RECOMBINATION; KNOCK-IN; CAS9; CRISPR/CAS9; MUTAGENESIS; SPECIFICITY; NUCLEASES; CLEAVAGE; REPAIR;

D O I：

10.1016/B978-0-12-801185-0.00019-2

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Our ability to modify the Drosophila genome has recently been revolutionized by the development of the CRISPR system. The simplicity and high efficiency of this system allows its widespread use for many different applications, greatly increasing the range of genome modification experiments that can be performed. Here, we first discuss some general design principles for genome engineering experiments in Drosophila and then present detailed protocols for the production of CRISPR reagents and screening strategies to detect successful genome modification events in both tissue culture cells and animals.

引用

页码：415 / 439

页数：25

共 50 条

[1] Genome editing using CRISPR/Cas9-based knock-in approaches in zebrafish
Albadri, Shahad
Del Bene, Filippo
Revenu, Celine
METHODS, 2017, 121 : 77 - 85
[2] Cas9-Based Genome Editing in Xenopus tropicalis
Nakayama, Takuya
Blitz, Ira L.
Fish, Margaret B.
Odeleye, Akinleye O.
Manohar, Sumanth
Cho, Ken W. Y.
Grainger, Robert M.
USE OF CRISPR/CAS9, ZFNS, AND TALENS IN GENERATING SITE-SPECIFIC GENOME ALTERATIONS, 2014, 546 : 355 - 375
[3] Cas9-Based Genome Editing in Zebrafish
Gonzales, Andrew P. W.
Yeh, Jing-Ruey Joanna
USE OF CRISPR/CAS9, ZFNS, AND TALENS IN GENERATING SITE-SPECIFIC GENOME ALTERATIONS, 2014, 546 : 377 - 413
[4] Guide RNA modification as a way to improve CRISPR/Cas9-based genome-editing systems
Filippova, Julia
Matveeva, Anastasiya
Zhuravlev, Evgenii
Stepanov, Grigory
BIOCHIMIE, 2019, 167 : 49 - 60
[5] CRISPR/Cas9-based genome editing in the era of CAR T cell immunotherapy
Salas-Mckee, January
Kong, Weimin
Gladney, Whitney L.
Jadlowsky, Julie K.
Plesa, Gabriela
Davis, Megan M.
Fraietta, Joseph A.
HUMAN VACCINES & IMMUNOTHERAPEUTICS, 2019, 15 (05) : 1126 - 1132
[6] Precision genome editing in plants: state-of-the-art in CRISPR/Cas9-based genome engineering
Wada, Naoki
Ueta, Risa
Osakabe, Yuriko
Osakabe, Keishi
BMC PLANT BIOLOGY, 2020, 20 (01)
[7] CRISPR/Cas9-based genome editing of the filamentous fungi: the state of the art
Shi, Tian-Qiong
Liu, Guan-Nan
Ji, Rong-Yu
Shi, Kun
Song, Ping
Ren, Lu-Jing
Huang, He
Ji, Xiao-Jun
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2017, 101 (20) : 7435 - 7443
[8] A CRISPR/Cas9-based genome editing system for Rhodococcus ruber TH
Liang, Youxiang
Jiao, Song
Wang, Miaomiao
Yu, Huimin
Shen, Zhongyao
METABOLIC ENGINEERING, 2020, 57 : 13 - 22
[9] CRISPR/Cas9-Based Genome Editing in Mice by Single Plasmid Injection
Fujihara, Yoshitaka
Ikawa, Masahito
USE OF CRISPR/CAS9, ZFNS, AND TALENS IN GENERATING SITE-SPECIFIC GENOME ALTERATIONS, 2014, 546 : 319 - 336
[10] Cas9-Based Tools for Targeted Genome Editing and Transcriptional Control
Xu, Tao
Li, Yongchao
Van Nostrand, Joy D.
He, Zhili
Zhou, Jizhong
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2014, 80 (05) : 1544 - 1552

← 1 2 3 4 5 →