Feasibility and effectiveness of endoscopic irreversible electroporation for the upper gastrointestinal tract: an experimental animal study

被引：10

作者：

Jeon, Han Jo ^{[1
]}

Choi, Hyuk Soon ^{[1
]}

Keum, Bora ^{[1
]}

Bang, Eun Joo ^{[1
]}

Lee, Kang Won ^{[1
]}

Kim, Sang Hyun ^{[1
]}

Yim, Sun Young ^{[1
]}

Lee, Jae Min ^{[1
]}

Kim, Eun Sun ^{[1
]}

Seo, Yeon Seok ^{[1
]}

Jeen, Yoon Tae ^{[1
]}

Lee, Hong Sik ^{[1
]}

Chun, Hoon Jai ^{[1
]}

Kim, Hong Bae ^{[2
]}

Kim, Jong Hyuk ^{[3
]}

机构：

[1] Korea Univ, Coll Med, Dept Internal Med, Div Gastroenterol & Hepatol, 73 Goryeodae Ro, Seoul 02841, South Korea

[2] Seoul Natl Univ, Dept Biosyst & Biomat Sci & Engn, Seoul, South Korea

[3] Univ Minnesota, Coll Vet Med, Dept Vet Clin Sci, St Paul, MN 55108 USA

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

基金：

新加坡国家研究基金会;

关键词：

ARGON PLASMA COAGULATION; RADIOFREQUENCY ABLATION; TISSUE ABLATION; GASTRIC-CANCER; IMPEDANCE; CELLS;

D O I：

10.1038/s41598-021-94583-w

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Irreversible electroporation (IRE) is a local non-thermal ablative technique currently used to treat solid tumors. Here, we investigated the clinical potency and safety of IRE with an endoscope in the upper gastrointestinal tract. Pigs were electroporated with recently designed endoscopic IRE catheters in the esophagus, stomach, and duodenum. Two successive strategies were introduced to optimize the electrical energy for the digestive tract. First, each organ was electroporated and the energy upscaled to confirm the upper limit energy inducing improper tissue results, including bleeding and perforation. Excluding the unacceptable energy from the first step, consecutive electroporations were performed with stepwise reductions in energy to identify the energy that damaged each layer. Inceptive research into inappropriate electrical intensity contributed to extensive hemorrhage and bowel perforation for each tissue above a certain energy threshold. However, experiments performed below the precluded energy accompanying hematoxylin and eosin staining and terminal deoxynucleotidyl transferase dUTP nick-end labeling assays showed that damaged mucosal area and depth significantly decreased with decreased energy. Relevant histopathology showed infiltration of inflammatory cells with pyknotic nuclei at the electroporated lesion. This investigation demonstrated the possibility of endoscopic IRE in mucosal dysplasia or early malignant tumors of the hollow viscus.

引用

页数：10

共 47 条

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