Identification of potential pancreatic cancer serum markers: Increased sialyl-Lewis X on ceruloplasmin

被引：44

作者：

Balmana, Meritxell ^{[1
]}

Sarrats, Ariadna ^{[1
]}

Llop, Esther ^{[1
]}

Barrabes, Silvia ^{[1
]}

Saldova, Radka ^{[2
]}

Ferri, Maria Jose ^{[3
]}

Figueras, Joan ^{[4
]}

Fort, Esther ^{[5
]}

de Llorens, Rafael ^{[1
]}

Rudd, Pauline M. ^{[2
,4
]}

Peracaula, Rosa ^{[1
]}

机构：

[1] Univ Girona, Dept Biol, Girona, Spain

[2] Natl Inst Bioproc Res & Training, NIBRT GlycoSci Grp, Dublin, Ireland

[3] Dr Josep Trueta Univ Hosp, Lab ICS Girona, Girona, Spain

[4] Dr Josep Trueta Univ Hosp, Dept Surg, IdIBGi, Girona, Spain

[5] Dr Josep Trueta Univ Hosp, Digest Unit, Girona, Spain

来源：

CLINICA CHIMICA ACTA | 2015年 / 442卷

关键词：

Acute-phase proteins; Biomarker; Ceruloplasmin; Pancreatic cancer; Pancreatitis; Sialyl-Lewis X; ACUTE-PHASE REACTION; GLYCOSYLATION ANALYSIS; GLYCOPROTEINS; EXPRESSION; CHROMATOGRAPHY; BIOMARKERS; PROTEINS; PATTERNS; ANTIGENS; BINDING;

D O I：

10.1016/j.cca.2015.01.007

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

Pancreatic adenocarcinoma (PDAC) usually shows an enhanced expression of sialyl-Lewis X (sLe(x)) and related epitopes. PDAC may secrete some of the proteins carrying such increased sLe(x) determinant into serum, so they could be used as PDAC markers. Previously, we identified acute-phase proteins with increased sLe(x) in both PDAC and in chronic pancreatitis patients. In this study, depleted sera from the main acute-phase proteins has been analysed for the search of proteins with increased sLe(x) levels in PDAC. Sera from healthy controls, chronic pancreatitis and PDAC patients were depleted, electrophoresed and subjected to sLe(x) immunodetection. Proteins that differentially expressed sLe(x) in PDAC were typsin digested and identified by LC-ESI-QTOF mass spectrometry. Five protein bands that differentially expressed sLe(x) in PDAC were identified and corresponded to seven different acute-phase proteins. Among them, ceruloplasmin (CP) was selected for further analysis. N-glycan sequencing of CP confirmed the increase of sLe(x) levels in CP in PDAC patients. Healthy controls, chronic pancreatitis and PDAC patients' sera were immunoprecipitated with anti-CP antibodies, and their sLe(x) and CP levels were analysed by western blot. The sLe(x)/CP ratio tended to be higher for the PDAC group, which altogether suggests that the sLe(x)/CP ratio could be a useful biomarker for PDAC. (C) 2015 Elsevier B.V. All rights reserved.

引用

页码：56 / 62

页数：7

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