Phytochemical screening and evaluation of antifungal activity from Dalbergia sissoo Roxb. stem

The present study was undertaken for qualitative tests for phytochemicals and evaluation of antifungal activity from stem of Shisham (Dalbergia sissoo Roxb.). The plant materials was collected from the university campus CCS, Haryana Agricultural University, Hisar. After this the plant materials were immediately brought to laboratory and were kept at room temperature under shade before processing. Result: It shows presence of various phytochemicals such as carbohydrates, sugars, saponins, proteins, flavanoids, triterpenoids and tannins etc. The maximum antifungal activity was found in chloroform fraction 98.23% growth inhibition at concentration 2000 mu g/ml, moderate antifungal activity was shown by ethyl acetate and benzene fractions with 88.23% and 80.00% growth inhibition against tested fungi. Methanol fraction of D. sissoo stem showed 72.94% growth inhibition at 2000 mu g/ml concentration followed by acetone and hexane fractions exhibited by 70.58% growth inhibition at 2000 mu g/ml against Rhizoctoniasolani fungi.