1H high-resolution magic angle spinning NMR spectroscopy for the investigation of a Ras lipopeptide in a lipid membrane

被引：0

作者：

Huster, D

Kuhn, K

Kadereit, D

Waldmann, H

Arnold, K

机构：

[1] Univ Leipzig, Inst Med Phys & Biophys, D-04103 Leipzig, Germany

[2] Max Planck Inst Mol Physiol, D-44227 Dortmund, Germany

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2001年 / 40卷 / 06期

关键词：

D O I：

10.1002/1521-3773(20010316)40:6<1056::AID-ANIE10560>3.0.CO;2-7

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

A double lipid-modified peptide which mimics the seven amino acids of the C terminus of the N-Ras protein has been investigated by high-resolution 1H magic angle spinning NMR spectroscopy. The axially symmetric motion of the peptide in the bilayer decreases the NMR line widthes, which facilitates signal assignment and localization of the peptide in the membrane (see schematic representation).

引用

收藏

页码：1056 / +

页数：4

相关论文

共 20 条

[1] Bioorganic synthesis of lipid-modified proteins for the study of signal transduction [J].

Bader, B ;

Kuhn, K ;

Owen, DJ ;

Waldmann, H ;

Wittinghofer, A ;

Kuhlmann, J .

NATURE, 2000, 403 (6766) :223-226

[2] High-speed magic angle spinning solid-state H-1 nuclear magnetic resonance study of the conformation of gramicidin a in lipid bilayers [J].

Bouchard, M ;

Davis, JH ;

Auger, M .

BIOPHYSICAL JOURNAL, 1995, 69 (05) :1933-1938

[3] PROTEIN LIPIDATION IN CELL SIGNALING [J].

CASEY, PJ .

SCIENCE, 1995, 268 (5208) :221-225

[4] Solid-state NMR and hydrogen-deuterium exchange in a bilayer-solubilized peptide: Structural and mechanistic implications [J].

Cotten, M ;

Fu, R ;

Cross, TA .

BIOPHYSICAL JOURNAL, 1999, 76 (03) :1179-1189

[5] High resolution H-1 nuclear magnetic resonance of a transmembrane peptide [J].

Davis, JH ;

Auger, M ;

Hodges, RS .

BIOPHYSICAL JOURNAL, 1995, 69 (05) :1917-1932

[6] Interpretation of NOESY cross-relaxation rates from molecular dynamics simulation of a lipid bilayer [J].

Feller, SE ;

Huster, D ;

Gawrisch, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1999, 121 (38) :8963-8964

[7] HIGH-FIELD, HIGH-RESOLUTION PROTON MAGIC-ANGLE SAMPLE-SPINNING NUCLEAR MAGNETIC-RESONANCE SPECTROSCOPIC STUDIES OF GEL AND LIQUID-CRYSTALLINE LIPID BILAYERS AND THE EFFECTS OF CHOLESTEROL [J].

FORBES, J ;

HUSTED, C ;

OLDFIELD, E .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1988, 110 (04) :1059-1065

[8]

Hinterding K, 1998, ANGEW CHEM INT EDIT, V37, P688, DOI 10.1002/(SICI)1521-3773(19980403)37:6<688::AID-ANIE688>3.0.CO

[9]

2-B

[10]

HINTERDING K, 1998, ANGEW CHEM, V110, P716