High Expression of lncRNA AFAP1-AS1 Promotes the Progression of Colon Cancer and Predicts Poor Prognosis

被引：79
作者：
Bo, Hao [1 ,2 ,3 ,4 ,5 ]
Fan, Liqing [5 ]
Li, Jingjing [6 ]
Liu, Zhizhong [7 ,8 ]
Zhang, Shanshan [1 ]
Shi, Lei [2 ,3 ,4 ]
Guo, Can [2 ,3 ,4 ]
Li, Xiayu [6 ]
Liao, Qianjin [2 ,3 ,4 ,7 ,8 ]
Zhang, Wenling [2 ,3 ,4 ]
Zhou, Ming [1 ,2 ,3 ,4 ]
Xiang, Bo [1 ,2 ,3 ,4 ]
Li, Xiaoling [1 ,2 ,3 ,4 ]
Li, Guiyuan [1 ,2 ,3 ,4 ]
Xiong, Wei [1 ,2 ,3 ,4 ]
Zeng, Zhaoyang [1 ,2 ,3 ,4 ]
Xiong, Fang [1 ,2 ,3 ,4 ]
Gong, Zhaojian [2 ,3 ,4 ,9 ]
机构：
[1] Cent South Univ, Key Lab Carcinogenesis, Chinese Minist Hlth, Xiangya Hosp, Changsha, Hunan, Peoples R China
[2] Cent South Univ, Key Lab Carcinogenesis, Changsha, Hunan, Peoples R China
[3] Cent South Univ, Canc Invas Chinese Minist Educ, Canc Res Inst, Changsha, Hunan, Peoples R China
[4] Cent South Univ, Sch Basic Med Sci, Changsha, Hunan, Peoples R China
[5] Cent South Univ, Sch Basic Med Sci, Inst Reprod & Stem Cell Engn, Changsha, Hunan, Peoples R China
[6] Cent South Univ, Dept Plast Surg, Hunan Key Lab Nonresolving Inflammat & Canc, Dis Genome Res Ctr,Xiangya Hosp 3, Changsha, Hunan, Peoples R China
[7] Cent South Univ, Hunan Key Lab Translat Radiat Oncol, Hunan Canc Hosp, Xiangya Sch Med, Changsha, Hunan, Peoples R China
[8] Cent South Univ, Affiliated Canc Hosp, Xiangya Sch Med, Changsha, Hunan, Peoples R China
[9] Cent South Univ, Xiangya Hosp 2, Dept Oral & Maxillofacial Surg, Changsha, Hunan, Peoples R China
来源：
JOURNAL OF CANCER | 2018年 / 9卷 / 24期
基金：
中国国家自然科学基金;
关键词：
long non-coding RNA (lncRNA); AFAP1 antisense RNA 1 (AFAP1-AS1); colon cancer (CC); metastasis; prognosis; LONG NONCODING RNA; METASTASIS; MIGRATION; OVEREXPRESSION; PROLIFERATION; CYTOSKELETON; EVOLUTION; INVASION; DATABASE;
D O I：
10.7150/jca.26461
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Long non-coding RNAs (lncRNAs) are dysregulated in various cancers. However, the clinical relevance and functional roles of AFAP1-AS1 in colon cancer (CC) have not been clarified. We analyzed the lncRNA expression patterns in Gene Expression Omnibus (GEO) datasets and the Cancer Genome Atlas (TCGA) RNA-seq datasets, and found that the expression level of AFAP1-AS1 was significantly elevated in CC tissues. High levels of AFAP1-AS1 were associated with poor disease-free survival and overall survival in CC patients. In vitro experiments demonstrated that AFAP1-AS1 knockdown significantly inhibited the cell invasive and migration capability in CC cell line HT-29. AFAP1-AS1 knockdown also increased the expression of E-cadherin and ZO-1 while inhibited the expression of Vimentin, MMP9, ZEB1 and beta-catenin, suggesting that AFAP1-AS1 is involved in the epithelial-mesenchymal transition (EMT) process of CC. Further studies confirmed that AFAP1-AS1 knockdown also affected the actin-cytokeratin signaling pathway. Thus, AFAP1-AS1 might be a potential novel diagnostic marker and therapeutic target for CC.
引用
收藏
页码：4677 / 4683
页数：7
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