Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography mass spectrometry

被引：85

作者：

Mills, GA

Walker, V ^{[1
]}

Mughal, H

机构：

[1] Southampton Gen Hosp, Dept Chem Pathol, Southampton SO16 6YD, Hants, England

[2] Univ Portsmouth, Sch Pharm & Biomed Sci, Portsmouth PO1 2DT, Hants, England

来源：

JOURNAL OF CHROMATOGRAPHY B | 1999年 / 723卷 / 1-2期

关键词：

solid-phase microextraction; trimethylamine; trimethylamine oxide;

D O I：

10.1016/S0378-4347(98)00542-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography-mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen-polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories. (C) 1999 Elsevier Science B.V. All rights reserved.

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页码：281 / 285

页数：5

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