Quantitative determination of trimethylamine in urine by solid-phase microextraction and gas chromatography mass spectrometry

被引：85
作者：
Mills, GA
Walker, V [1 ]
Mughal, H
机构：
[1] Southampton Gen Hosp, Dept Chem Pathol, Southampton SO16 6YD, Hants, England
[2] Univ Portsmouth, Sch Pharm & Biomed Sci, Portsmouth PO1 2DT, Hants, England
来源：
JOURNAL OF CHROMATOGRAPHY B | 1999年 / 723卷 / 1-2期
关键词：
solid-phase microextraction; trimethylamine; trimethylamine oxide;
D O I：
10.1016/S0378-4347(98)00542-8
中图分类号：
Q5 [生物化学];
学科分类号：
071010 ; 081704 ;
摘要：
Trimethylaminuria (fish odour syndrome) is diagnosed from an increase in urinary excretion of trimethylamine with decreased trimethylamine oxide. We report a new quantitative stable isotope dilution gas chromatography-mass spectrometry procedure for the analysis of these metabolites using solid-phase microextraction (SPME). Both polydimethylsiloxane and mixed Carboxen-polydimethylsiloxane SPME fibres were found to be suitable for the headspace extraction of TMA. This new sampling technique could have wide application for the analysis of volatile and semi-volatile compounds by metabolic screening laboratories. (C) 1999 Elsevier Science B.V. All rights reserved.
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收藏
页码：281 / 285
页数：5
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