In Vivo Imaging of Glial Activation after Unilateral Labyrinthectomy in the Rat: A [18F]GE180-PET study

被引：15
作者：
Zwergal, Andreas [1 ,2 ]
Guenther, Lisa [1 ]
Brendel, Matthias [3 ,4 ]
Beck, Roswitha [1 ]
Lindner, Simon [3 ]
Xiong, Guoming [1 ]
Eilles, Eva [1 ]
Unterrainer, Marcus [3 ]
Albert, Nathalie Lisa [3 ]
Becker-Bense, Sandra [1 ,2 ]
Brandt, Thomas [1 ,5 ]
Ziegler, Sibylle [3 ]
la Fougere, Christian [1 ,6 ]
Dieterich, Marianne [1 ,2 ,4 ]
Bartenstein, Peter [1 ,3 ,4 ]
机构：
[1] Ludwig Maximilians Univ Munchen, German Ctr Vertigo & Balance Disorders, DSGZ, Munich, Germany
[2] Ludwig Maximilians Univ Munchen, Dept Neurol, Munich, Germany
[3] Ludwig Maximilians Univ Munchen, Dept Nucl Med, Munich, Germany
[4] SyNergy, Munich Cluster Syst Neurol, Munich, Germany
[5] Ludwig Maximilians Univ Munchen, Clin Neurosci, Munich, Germany
[6] Eberhard Karls Univ Tubingen, Dept Nucl Med, Tubingen, Germany
来源：
FRONTIERS IN NEUROLOGY | 2017年 / 8卷
关键词：
glial activation; vestibular compensation; small animal PET; translocator protein imaging; acute unilateral vestibulopathy; PERIPHERAL BENZODIAZEPINE-RECEPTOR; VESTIBULAR NUCLEUS NEURONS; 18 KDA TSPO; MICROGLIAL ACTIVATION; COMPENSATION; INJURY; MODEL; DEGENERATION; REGENERATION; ASTROCYTES;
D O I：
10.3389/fneur.2017.00665
中图分类号：
R74 [神经病学与精神病学];
学科分类号：
摘要：
The functional relevance of reactive gliosis for recovery from acute unilateral vestibulopathy is unknown. In the present study, glial activation was visualized in vivo by [F-18]GE180-PET in a rat model of unilateral labyrinthectomy (UL) and compared to behavioral vestibular compensation (VC) overtime. 14 Sprague-Dawley rats underwent a UL by transtympanic injection of bupivacaine/arsenilate, 14 rats a SHAM UL (injection of normal saline). Glial activation was depicted with [F-18]GE180-PET and ex vivo autoradiography at baseline and 7, 15, 30 days after UL/SHAM UL. Postural asymmetry and nystagmus were registered at 1, 2, 3, 7, 15, 30 days after UL/SHAM UL. Signs of vestibular imbalance were found only after UL, which significantly decreased until days 15 and 30. In parallel, [F-18]GE180-PET and ex vivo autoradiography depicted glial activation in the ipsilesional vestibular nerve and nucleus on days 7 and 15 after UL. Correlation analysis revealed a strong negative association of [F-18]GE180 uptake in the ipsilesional vestibular nucleus on day 7 with the rate of postural recovery (R = -0.90, p < 0.001), suggesting that glial activation accelerates VC. In conclusion, glial activation takes place in the ipsilesional vestibular nerve and nucleus within the first 30 days after UL in the rat and can be visualized in vivo by [F-18]GE180-PET.
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页数：10
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