Differential regulation of neuropeptide Y receptors in the brains of NPY knock-out mice

被引：31
|
作者：
Trivedi, PG [1 ]
Yu, H [1 ]
Trumbauer, M [1 ]
Chen, H [1 ]
Van der Ploeg, LHT [1 ]
Guan, XM [1 ]
机构：
[1] Merck Res Labs, Dept Obes Res, Rahway, NJ 07065 USA
来源：
PEPTIDES | 2001年 / 22卷 / 03期
关键词：
NPY receptor regulation; expression; mRNA; receptor binding; NPY knockout mouse;
D O I：
10.1016/S0196-9781(01)00349-7
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
To study the effect of NPY deletion on the regulation of its receptors in the NPY knockout (NPY KO) mice, the expression and binding of NPY receptors were investigated by in situ hybridization and receptor autoradiography using I-125-[Leu(31),Pro(34)]PYY and I-125-PYY3-36 as radioligands. A 6-fold increase in Y2 receptor mRNA was observed in the CA1 region of the hippocampus in NPY KO mice, but a significant change could not be detected for Y1, Y4, Y5 and y6 receptors. Receptor binding reveals a 60-400% increase of Y2 receptor binding in multiple brain areas. A similar increase in Y1 receptor binding was seen only in the hypothalamus. These results demonstrate the NPY receptor expression is altered in mice deficient for its natural ligand. (C) 2001 Elsevier Science Inc. All rights reserved.
引用
收藏
页码：395 / 403
页数：9
相关论文
共 50 条
  • [1] Knock-out mice reveal a critical antiepileptic role for neuropeptide Y
    Baraban, SC
    Hollopeter, G
    Erickson, JC
    Schwartzkroin, PA
    Palmiter, RD
    JOURNAL OF NEUROSCIENCE, 1997, 17 (23): : 8927 - 8936
  • [2] Regulation of neuropeptide gene expression by fasting in NPY-Y1 receptor knock out mice
    Niki, T
    Shiiya, T
    Higuchi, H
    JOURNAL OF PHARMACOLOGICAL SCIENCES, 2006, 100 : 191P - 191P
  • [3] Generation and study of AgRP/NPY double knock-out mice
    Trumbauer, M
    Weingarth, D
    Qian, S
    Marsh, D
    Guan, XM
    Yu, H
    Frazier, E
    Chen, A
    Novi, D
    Feng, Y
    Camacho, R
    Kuca, S
    Shearman, L
    Metzger, J
    Gopal-Truter, S
    Van der Ploeg, L
    Chen, H
    OBESITY RESEARCH, 2001, 9 : 104S - 104S
  • [4] Differential gene expression in brains from knock-out mice using DNA microarrays
    Potier, MC
    Golfier, G
    Gibelin, N
    Lambolez, B
    Cauli, B
    Rossier, J
    JOURNAL OF NEUROCHEMISTRY, 2001, 78 : 110 - 110
  • [5] Neuropeptide Y (NPY) and NPY receptors in the cardiovascular system: implication in the regulation of intracellular calcium
    Jacques, Danielle
    Abdel-Samad, Dirna
    CANADIAN JOURNAL OF PHYSIOLOGY AND PHARMACOLOGY, 2007, 85 (01) : 43 - 53
  • [6] Regulation of insulin secretion in annexin VII (-/-) knock-out mice
    Ullrich, S
    Herr, C
    Hescheler, J
    Noegel, A
    DIABETOLOGIA, 2000, 43 : A134 - A134
  • [7] Nicotinic receptors subunits and nicotine reinforcement investigated with knock-out mice
    Picciotto, MR
    Zoli, M
    Rimondi, R
    Léna, C
    Marubio, LM
    Pich, EM
    Fuxe, K
    Changeux, JP
    EUROPEAN JOURNAL OF NEUROSCIENCE, 1998, 10 : 212 - 212
  • [8] Neuropeptide Y (NPY) and NPY receptors in the rat pineal gland
    Mikkelsen, JD
    Hauser, F
    Olcese, J
    MELATONIN AFTER FOUR DECADES: AN ASSESSMENT OF ITS POTENTIAL, 1999, 460 : 95 - 107
  • [9] Knock-out and Knock-in Mice to Investigate the Role of Nicotinic Receptors in the Central Nervous System
    Champtiaux, N.
    Changeux, J. P.
    CNS & NEUROLOGICAL DISORDERS-DRUG TARGETS, 2002, 1 (04) : 319 - 330
  • [10] Solving hemochromatosis with knock-out mice
    Pietrangelo, A
    JOURNAL OF HEPATOLOGY, 2003, 38 (01) : 121 - 123
12345