Inhibition of human immunodeficiency virus type-1 (HIV-1) replication at a reverse transcription step by human cell factor(s)

被引：2

作者：

Tempaku, A ^{[1
]}

机构：

[1] Univ Fukui, Fac Med Sci, Sch Med, Fukui 9101193, Japan

来源：

BIOLOGICAL & PHARMACEUTICAL BULLETIN | 2005年 / 28卷 / 05期

关键词：

human immunodeficiency virus type-1 (HIV-1); reverse transcriptase; host factor;

D O I：

10.1248/bpb.28.893

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

Infection of human cell with human immunodeficiency virus type-1 (HIV-1) was suppressed by cellular genetic factor(s) at reverse transcription step. Although same amount of virus adsorbed on both cells, small amount of HIV-1 (11113 strain) infected HeLa (MAGI/CCR5) cell, while large amount of RIV-1 infected HOS (GHOST/CXCR4) cell. Regulation of virus replication at postentry level by cellular factor(s) had an important role for low efficiency of RIV-1 infection to MAGI/CCR5 cell. Provirus DNA formation in MAGI/CCR5 cell was less efficient than in GHOST/CXCR4 cell. Once GHOST/CXCR4 cell was fused with MAGI/CCR5 cell, susceptibility against HIV-1 decreased. Further, HIV-1 reverse transcriptase (RT) activity was strongly inhibited by cytosolic protein, derived from MAGI/CCR5 cell, in vitro. This research cleared a certain human cell genetically carries some factor(s) which inhibits the activity of HIV-1 RT.

引用

页码：893 / 897

页数：5

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