Unusual change in molecular weight of polyhydroxyalkanoate (PHA) during cultivation of PHA-accumulating Escherichia coli

被引：21

作者：

Agus, Jumiarti ^{[1
]}

Kahar, Prihardi ^{[2
]}

Hyakutake, Manami ^{[1
]}

Tomizawa, Satoshi ^{[1
]}

Abe, Hideki ^{[3
]}

Tsuge, Takeharu ^{[1
]}

Satoh, Yasuharu ^{[4
]}

Tajima, Kenji ^{[4
]}

机构：

[1] Tokyo Inst Technol, Dept Innovat & Engineered Mat, Midori Ku, Yokohama, Kanagawa 2268502, Japan

[2] Meisei Univ, Asian Ctr Environm Res, Hino, Tokyo 1918506, Japan

[3] RIKEN, Biomass Engn Program, Bioplast Res Team, Wako, Saitama 3510198, Japan

[4] Hokkaido Univ, Grad Sch Engn, Div Biotechnol & Macromol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan

来源：

POLYMER DEGRADATION AND STABILITY | 2010年 / 95卷 / 12期

关键词：

Polyhydroxyalkanoate; PHA synthase; Bacillus; Molecular weight; Random scission; PSEUDOMONAS SP 61-3; BACILLUS SP INT005; THERMAL-DEGRADATION; SYNTHASE; BACTERIA; BIOSYNTHESIS; SOIL;

D O I：

10.1016/j.polymdegradstab.2010.09.009

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

This study aimed to investigate the factors affecting molecular weight of poly[(R)-3-hydroxybutyrate] [P(3HB)] when polyhydroxyalkanoate (PHA) synthase (PhaRC(Bsp)) from Bacillus sp. INT005 was used for P(3HB) synthesis in Escherichia coli JM109. It was found that the molecular weight of P(3HB) decreased with time in mid- and late-phase of culture and was strongly affected by culture temperature. At 37 degrees C culture temperature, the molecular weight of P(3HB) rapidly decreased from 4.4 x 10(5) to 4.8 x 10(4) with culture time, whereas it was almost unchanged at 25 degrees C. Kinetic analysis suggested that the decrease in molecular weight of P(3HB) was due to random scission of the polymer chain. The decrease in molecular weight of P(3HB) was not observed when PHA synthases other than PhaRC(Bsp) were expressed. This study sheds light on the unique behaviour in molecular weight change of P(3HB) that is synthesized by E. coli expressing PhaRCB(sp). (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2250 / 2254

页数：5

共 18 条

[11] Polyhydroxyalkanoate synthase from Bacillus sp INT005 is composed of PhaC and PhaR [J].

Satoh, Y ;

Minamoto, N ;

Tajima, K ;

Munekata, M .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2002, 94 (04) :343-350

[12] Synthesis, structure and properties of polyhydroxyalkanoates: biological polyesters [J].

Sudesh, K ;

Abe, H ;

Doi, Y .

PROGRESS IN POLYMER SCIENCE, 2000, 25 (10) :1503-1555

[13] Isolation and characterization of Bacillus sp INT005 accumulating polyhydroxyalkanoate (PHA) from gas field soil [J].

Tajima, K ;

Igari, T ;

Nishimura, D ;

Nakamura, M ;

Satoh, Y ;

Munekata, M .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2003, 95 (01) :77-81

[14] Synthesis, properties and uses of bacterial storage lipid granules as naturally occurring nanoparticles [J].

Thomson, Nicholas ;

Summers, David ;

Sivaniah, Easan .

SOFT MATTER, 2010, 6 (17) :4045-4057

[15] An extra large insertion in the polyhydroxyalkanoate synthase from Delftia acidovorans DS-17:: its deletion effects and relation to cellular proteolysis [J].

Tsuge, T ;

Imazu, S ;

Takase, K ;

Taguchi, S ;

Doi, Y .

FEMS MICROBIOLOGY LETTERS, 2004, 231 (01) :77-83

[16] Metabolic improvements and use of inexpensive carbon sources in microbial production of polyhydroxyalkanoates [J].

Tsuge, T .

JOURNAL OF BIOSCIENCE AND BIOENGINEERING, 2002, 94 (06) :579-584

[17] Thiolysis of poly(3-hydroxybutyrate) with polyhydroxyalkanoate synthase from Ralstonia eutropha [J].

Uchino, K ;

Saito, T .

JOURNAL OF BIOCHEMISTRY, 2006, 139 (03) :615-621

[18] Polyhydroxyalkanoates in Gram-positive bacteria: insights from the genera Bacillus and Streptomyces [J].

Valappil, Sabeel P. ;

Boccaccini, Aldo R. ;

Bucke, Christopher ;

Roy, Ipsita .

ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY, 2007, 91 (01) :1-17

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