Unusual change in molecular weight of polyhydroxyalkanoate (PHA) during cultivation of PHA-accumulating Escherichia coli

被引：21

作者：

Agus, Jumiarti ^{[1
]}

Kahar, Prihardi ^{[2
]}

Hyakutake, Manami ^{[1
]}

Tomizawa, Satoshi ^{[1
]}

Abe, Hideki ^{[3
]}

Tsuge, Takeharu ^{[1
]}

Satoh, Yasuharu ^{[4
]}

Tajima, Kenji ^{[4
]}

机构：

[1] Tokyo Inst Technol, Dept Innovat & Engineered Mat, Midori Ku, Yokohama, Kanagawa 2268502, Japan

[2] Meisei Univ, Asian Ctr Environm Res, Hino, Tokyo 1918506, Japan

[3] RIKEN, Biomass Engn Program, Bioplast Res Team, Wako, Saitama 3510198, Japan

[4] Hokkaido Univ, Grad Sch Engn, Div Biotechnol & Macromol Chem, Kita Ku, Sapporo, Hokkaido 0608628, Japan

来源：

POLYMER DEGRADATION AND STABILITY | 2010年 / 95卷 / 12期

关键词：

Polyhydroxyalkanoate; PHA synthase; Bacillus; Molecular weight; Random scission; PSEUDOMONAS SP 61-3; BACILLUS SP INT005; THERMAL-DEGRADATION; SYNTHASE; BACTERIA; BIOSYNTHESIS; SOIL;

D O I：

10.1016/j.polymdegradstab.2010.09.009

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

This study aimed to investigate the factors affecting molecular weight of poly[(R)-3-hydroxybutyrate] [P(3HB)] when polyhydroxyalkanoate (PHA) synthase (PhaRC(Bsp)) from Bacillus sp. INT005 was used for P(3HB) synthesis in Escherichia coli JM109. It was found that the molecular weight of P(3HB) decreased with time in mid- and late-phase of culture and was strongly affected by culture temperature. At 37 degrees C culture temperature, the molecular weight of P(3HB) rapidly decreased from 4.4 x 10(5) to 4.8 x 10(4) with culture time, whereas it was almost unchanged at 25 degrees C. Kinetic analysis suggested that the decrease in molecular weight of P(3HB) was due to random scission of the polymer chain. The decrease in molecular weight of P(3HB) was not observed when PHA synthases other than PhaRC(Bsp) were expressed. This study sheds light on the unique behaviour in molecular weight change of P(3HB) that is synthesized by E. coli expressing PhaRCB(sp). (C) 2010 Elsevier Ltd. All rights reserved.

引用

页码：2250 / 2254

页数：5

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