Ageing alters the production of nitric oxide and prostanoids after IL-1β exposure in mesenteric resistance arteries

被引:26
作者
Briones, AM
Salaices, M
Vila, E [1 ]
机构
[1] Univ Autonoma Barcelona, Fac Med, Dpt Farmacol Terapeut & Toxicol, E-08193 Barcelona, Spain
[2] Univ Autonoma Madrid, Fac Med, Dpt Farmacol & Terapeut, E-28029 Madrid, Spain
关键词
iNOS; COX-2; resistance arteries; ageing;
D O I
10.1016/j.mad.2005.01.006
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
We aimed to analyse age influence on the production of inflammatory mediators from inducible isoforms of nitric oxide synthase (iNOS) and cyclooxygenase (COX-2) in rat mesenteric resistance arteries (MRA). The second and/or third branches of MRA from young (3-month-old) and old (22-month-old) male Sprague-Dawley rats were incubated in culture medium with or without interleukin-1 beta (IL-1 beta; 10 ng/ml, 14 h). IL-1 beta did not modify endothelial NOS (eNOS) expression or endothelial cell distribution. However, IL-1 beta increased nitrite production and iNOS expression in endothelial and smooth muscle cells more in arteries from young than from old rats. IL-1 beta also increased PG12 levels and COX-2 expression in the three layers of the vascular wall. Ageing did not affect COX-2 expression but did increase TXA(2) and PGF(2 alpha) levels. The maximum contraction to phenylephrine was increased in arteries from old rats after IL-1 beta treatment. Inhibition of iNOS and COX-2 with 1400 W and NS398, respectively, abolished the differences in phenylephrine contraction. In conclusion, IL-1 beta induced an inflammatory response in MRA with associated increases in iNOS and COX-2 expression. The lower increase in nitrite production from iNOS together with a greater contractile prostanoid production in the old rats would be responsible for the increase observed in their contraction to phenylephrine after IL-1 beta treatment. (c) 2005 Elsevier Ireland Ltd. All rights reserved.
引用
收藏
页码:710 / 721
页数:12
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