The synthesis and characterisation of a novel dendritic system for gene delivery

被引：26

作者：

Coles, Daniel J. ^{[1
]}

Yang, Shu ^{[2
]}

Esposito, Anna ^{[1
]}

Mitchell, Deanne ^{[2
]}

Minchin, Rodney F. ^{[2
]}

Toth, Istvan ^{[1
]}

机构：

[1] Univ Queensland, Sch Mol & Microbial Sci, St Lucia, Qld 4072, Australia

[2] Univ Queensland, Sch Biomed Sci, St Lucia, Qld 4072, Australia

来源：

TETRAHEDRON | 2007年 / 63卷 / 49期

关键词：

gene delivery; dendrimer; TAT; NLS;

D O I：

10.1016/j.tet.2007.09.048

中图分类号：

O62 [有机化学];

学科分类号：

070303 ; 081704 ;

摘要：

There has been increasing interest in recent years in gene delivery. We report the synthesis of non-viral delivery systems composed of variations of the cell penetrating peptide TAT, a nuclear localisation signal peptide and dendritic polylysine. The delivery systems were tested for their ability to form complexes with plasmid DNA by utilising gel shift analysis, isothermal titration calorimetry, particle size analysis, zeta potential and transmission electron microscopy. These techniques indicated the successful formation of complexes between the peptide dendrimer and DNA. (c) 2007 Elsevier Ltd. All rights reserved.

引用

页码：12207 / 12214

页数：8

共 34 条

[1] The delivery of antisense therapeutics [J].

Akhtar, S ;

Hughes, MD ;

Khan, A ;

Bibby, M ;

Hussain, M ;

Nawaz, Q ;

Double, J ;

Sayyed, P .

ADVANCED DRUG DELIVERY REVIEWS, 2000, 44 (01) :3-21

[2] Evaluation of cell-penetrating peptides (CPPs) as vehicles for intracellular delivery of antisense peptide nucleic acid (PNA) [J].

Bendifallah, Nadia ;

Rasmussen, Frank Winther ;

Zachar, Vladimir ;

Ebbesen, Peter ;

Nielsen, Peter E. ;

Koppelhus, Uffe .

BIOCONJUGATE CHEMISTRY, 2006, 17 (03) :750-758

[3] Lipid, sugar and liposaccharide based delivery systems 2 [J].

Blanchfield, J ;

Toth, I .

CURRENT MEDICINAL CHEMISTRY, 2004, 11 (17) :2375-2382

[4] Targeting of a cholecystokinin-DNA complex to pancreatic cells in vitro and in vivo [J].

Carpenter, DS ;

Minchin, RF .

GENE THERAPY, 1998, 5 (06) :848-854

[5] The use of synthetic polymers for delivery of therapeutic antisense oligodeoxynucleotides [J].

Chirila, TV ;

Rakoczy, PE ;

Garrett, KL ;

Lou, X ;

Constable, IJ .

BIOMATERIALS, 2002, 23 (02) :321-342

[6]

Edwards RJ, 1996, GENE THER, V3, P937

[7] Thermodynamic analysis of polycation-DNA interaction applying titration microcalorimetry [J].

Ehtezazi, T ;

Rungsardthong, U ;

Stolnik, S .

LANGMUIR, 2003, 19 (22) :9387-9394

[8] Polymers for DNA delivery [J].

Eliyahu, H ;

Barenholz, Y ;

Domb, AJ .

MOLECULES, 2005, 10 (01) :34-64

[9] Membrane-permeable arginine-rich peptides and the translocation mechanisms [J].

Futaki, S .

ADVANCED DRUG DELIVERY REVIEWS, 2005, 57 (04) :547-558

[10] Oligoarginine vectors for intracellular delivery: Design and cellular-uptake mechanisms [J].

Futaki, S .

BIOPOLYMERS, 2006, 84 (03) :241-249

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