Cloning of human ENC-1 and evaluation of its expression and regulation in nervous system tumors

被引：42

作者：

Hernandez, MC ^{[1
]}

Andres-Barquin, PJ ^{[1
]}

Holt, I ^{[1
]}

Israel, MA ^{[1
]}

机构：

[1] Univ Calif San Francisco, Sch Med, Brain Tumor Res Ctr, Dept Neurol Surg,Preuss Lab Mol Neurooncol, San Francisco, CA 94143 USA

来源：

EXPERIMENTAL CELL RESEARCH | 1998年 / 242卷 / 02期

关键词：

human ENC-1; neuroblastoma; differentiation; nervous system; actin-binding protein;

D O I：

10.1006/excr.1998.4109

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

We recently identified and characterized a novel murine gene, ENC-1, that is expressed primarily in the nervous system and encodes an actin-binding protein. To gain insight into a potential role for ENC-1 gene in the processes of cell differentiation and malignant transformation in the human nervous system, we first cloned and characterized the human homologue of ENC-1. The human ENC-1 gene appeared to be highly expressed in adult brain and spinal cord, and in a number of cell lines derived from nervous system tumors we detected low steady-state levels of ENC-1 mRNA. We used a neuroblastoma differentiation model, the retinoic acid-induced neuronal differentiation of SMS-KCNR cells, to study the regulation of the ENC-1 gene during neural crest cell differentiation. We found that the expression of ENC-1 increased dramatically in the differentiated SMS-KCNR cells as compared to control undifferentiated cells. These results suggest that ENC-1 expression plays a role during differentiation of neural crest cells and may be down regulated in neuroblastoma tumors.. (C) 1998 Academic Press.

引用

页码：470 / 477

页数：8

共 36 条

[1] HUMAN NEURO-BLASTOMA CELL-LINES AS MODELS FOR THE INVITRO STUDY OF NEOPLASTIC AND NEURONAL CELL-DIFFERENTIATION [J].