A high density microelectrode array biosensor for detection of E-coli O157:H7

被引：288

作者：

Radke, SA ^{[1
]}

Alocilja, EC ^{[1
]}

机构：

[1] Michigan State Univ, E Lansing, MI 48823 USA

来源：

BIOSENSORS & BIOELECTRONICS | 2005年 / 20卷 / 08期

关键词：

microfabrication; pathogen; bacteria; electrode; antibody;

D O I：

10.1016/j.bios.2004.07.021

中图分类号：

Q6 [生物物理学];

学科分类号：

071011 ;

摘要：

A high density microelectrode array biosensor was developed for the detection of Escherichia coli 0 157: HT The biosensor was fabricated from (100) silicon with a 2 mum layer of thermal oxide as an insulating layer, an active area of 9.6 mm(2) and consists of an interdigitated gold electrode array. The sensor surface was functionalised for bacterial detection using heterobifunctional crosslinkers and immobilised polyclonal antibodies to create a biological sensing surface. Bacteria suspended in solution became attached to the immobilised antibodies when the biosensor was tested in liquid samples. The change in impedance caused by the bacteria was measured over a frequency range of 100 Hz-10 MHz. The biosensor was evaluated for E. coli O157:H7 detection in pure culture and inoculated food samples. The biosensor was able to discriminate between cellular concentrations of 10(4)-10(7) CFU/mL and has applications in detecting pathogens in food samples. (C) 2004 Elsevier B.V. All rights reserved.

引用

页码：1662 / 1667

页数：6

共 22 条

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