CCN2/CTGF silencing blocks cell aggregation in embryonal carcinoma P19 cell

被引：4
作者：
Aguiar, D. P. [1 ]
Coelho-Aguiar, J. M. [1 ]
Abreu, J. G. [1 ]
机构：
[1] Univ Fed Rio de Janeiro, Inst Ciencias Biomed, Programa Biol Celular & Desenvolvimento, BR-21949590 Rio de Janeiro, Brazil
来源：
BRAZILIAN JOURNAL OF MEDICAL AND BIOLOGICAL RESEARCH | 2011年 / 44卷 / 03期
关键词：
Teratocarcinoma; Adhesion; Condensation; CCN family; CCN2/CTGF; Extracellular matrix; TISSUE-GROWTH-FACTOR; FACTOR CTGF; TRANSCRIPTION FACTOR; BREAST-CANCER; RETINOIC ACID; UP-REGULATION; IN-VITRO; DIFFERENTIATION; MIGRATION; ANGIOGENESIS;
D O I：
10.1590/S0100-879X2011007500019
中图分类号：
Q [生物科学];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Connective tissue growth factor (CCN2/CTGF) is a matricellular-secreted protein involved in extracellular matrix remodeling. The P19 cell line is an embryonic carcinoma line widely used as a cellular model for differentiation and migration studies. In the present study, we employed an exogenous source of CCN2 and small interference RNA to address the role of CCN2 in the P19 cell aggregation phenomenon. Our data showed that increasing CCN2 protein concentrations from 0.1 to 20 nM decreased the number of cell clusters and dramatically increased cluster size without changing proliferation or cell survival, suggesting that CCN2 induced aggregation. In addition, CCN2 specific silencing inhibited typical P19 cell aggregation, which could be partially rescued by 20 nM CCN2. The present study demonstrates that CCN2 is a key molecule for cell aggregation of embryonic P19 cells.
引用
收藏
页码：200 / 205
页数：6
相关论文
共 40 条
[1]   Chordin-like CR domains and the regulation of evolutionarily conserved extracellular signaling systems [J].
Abreu, JG ;
Coffinier, C ;
Larraín, J ;
Oelgeschläger, M ;
De Robertis, EM .
GENE, 2002, 287 (1-2) :39-47
[2]   Connective-tissue growth factor (CTGF) modulates cell signalling by BMP and TGF-β [J].
Abreu, JG ;
Ketpura, NI ;
Reversade, B ;
De Robertis, EM .
NATURE CELL BIOLOGY, 2002, 4 (08) :599-604
[3]   Connective tissue growth factor-specific antibody attenuates tumor growth, metastasis, and angiogenesis in an orthotopic mouse model of pancreatic cancer [J].
Aikawa, Takuma ;
Gunn, Jason ;
Spong, Suzanne M. ;
Klaus, Stephen J. ;
Korc, Murray .
MOLECULAR CANCER THERAPEUTICS, 2006, 5 (05) :1108-1116
[4]   Isoquercitrin isolated from Hyptis fasciculata reduces glioblastoma cell proliferation and changes β-catenin cellular localization [J].
Amado, Nathalia G. ;
Cerqueira, Debora M. ;
Menezes, Fabio S. ;
Mendes da Silva, Joaquim Fernando ;
Neto, Vivaldo Moura ;
Abreu, Jose G. .
ANTI-CANCER DRUGS, 2009, 20 (07) :543-552
[5]   N-terminal domains of CCN family 2/connective tissue growth factor bind to aggrecan [J].
Aoyama, Eriko ;
Hattori, Takako ;
Hoshijima, Mitsuhiro ;
Araki, Daisuke ;
Nishida, Takashi ;
Kubota, Satoshi ;
Takigawa, Masaharu .
BIOCHEMICAL JOURNAL, 2009, 420 :413-420
[6]  
Babic AM, 1999, MOL CELL BIOL, V19, P2958
[7]   THE MODULAR ARCHITECTURE OF A NEW FAMILY OF GROWTH-REGULATORS RELATED TO CONNECTIVE-TISSUE GROWTH-FACTOR [J].
BORK, P .
FEBS LETTERS, 1993, 327 (02) :125-130
[8]   Strategies for blocking the fibrogenic actions of connective tissue growth factor (CCN2): From pharmacological inhibition in vitro to targeted siRNA therapy in vivo [J].
Brigstock, David R. .
JOURNAL OF CELL COMMUNICATION AND SIGNALING, 2009, 3 (01) :5-18
[9]   Mechanical regulation of the Cyr61/CCN1 and CTGF/CCN2 proteins - Implications in mechanical stress-associated pathologies [J].
Chaqour, Brahim ;
Goppelt-Struebe, Margarete .
FEBS JOURNAL, 2006, 273 (16) :3639-3649
[10]  
Chen Xin-Ming, 2009, Front Biosci (Schol Ed), V1, P132, DOI 10.2741/s13
1234