BTI1, an Azoreductase with pH-Dependent Substrate Specificity

被引：27
作者：
Johansson, Hans E. [1 ]
Johansson, Mary K. [1 ]
Wong, Albert C. [1 ]
Armstrong, Eliana S. [1 ]
Peterson, Erik J. [1 ]
Grant, Richard E. [1 ]
Roy, Margaret A. [1 ]
Reddington, Mark V. [1 ]
Cook, Ronald M. [1 ]
机构：
[1] Biosearch Technol Inc, Novato, CA 94949 USA
来源：
APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2011年 / 77卷 / 12期
关键词：
ENZYMATIC REDUCTION; MOLECULAR-CLONING; NADPH; AZO;
D O I：
10.1128/AEM.02289-10
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The group II azoreductase BTI1 utilizes NADPH to directly cleave azo bonds in water-soluble azo dyes, including quenchers of fluorescence. Unexpectedly, optimal reduction was dye specific, ranging from a pH of < 5.5 for Janus green B, to pH 6.0 for methyl red, methyl orange, and BHQ-10, to pH > 8.3 for flame orange.
引用
收藏
页码：4223 / 4225
页数：3
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