Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma

被引:5
作者
Chen, Ping [1 ]
Zhong, Qi [1 ]
Li, Zufei [1 ]
Zhang, Yang [1 ]
Huang, Zhigang [1 ]
机构
[1] Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dongjiaomin Alley, Beijing 100730, Peoples R China
基金
中国国家自然科学基金;
关键词
basic transcription factor 3; head and neck squamous cell carcinomas; small interfering RNA; cell function; POLYPEPTIDE-ASSOCIATED COMPLEX; GENE-EXPRESSION; BETA-NAC; PROTEIN; RNA; APOPTOSIS; IDENTIFICATION; CHAPERONES;
D O I
10.3892/ol.2018.9699
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
Basic transcription factor 3 (BTF3), a transcription factor and modulator of apoptosis, is differentially expressed in carcinoma. To acquire further understanding of the involvement of BTF3 in carcinoma, the present study analyzed the expression of BTF3, as well as its role in cell function in nasopharyngeal carcinoma (NPC). BTF3 transcription rates in human NPC samples (n=46) and adjacent normal tissue samples (n=46) were analyzed using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry. BTF3-silencing in NPC cells was performed via specific small interfering RNA molecules. The function of BTF3 was analyzed by proliferation assays and colony forming assays using a Cellomic assay system. The positive expression rates of BTF3 were significantly increased in cancerous tissues compared with those in adjacent tissues (P<0.05). In addition, BTF3-silencing decreased cell proliferation and colony formation (P<0.01) in TCA-8113 and 5-8F cells. BTF3 is overexpressed in NPC, and its silencing is associated with decreased cell proliferation and colony formation, enhanced apoptosis and cell cycle regulation of TCA-8113 and 5-8F cells.
引用
收藏
页码:789 / 796
页数:8
相关论文
共 36 条
[21]   Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method [J].
Livak, KJ ;
Schmittgen, TD .
METHODS, 2001, 25 (04) :402-408
[22]   Proteomic studies on low- and high-grade human brain astrocytomas [J].
Odreman, F ;
Vindigni, M ;
Gonzales, ML ;
Niccolini, B ;
Candiano, G ;
Zanotti, B ;
Skrap, M ;
Pizzolitto, S ;
Stanta, G ;
Vindigni, A .
JOURNAL OF PROTEOME RESEARCH, 2005, 4 (03) :698-708
[23]   Eph receptors and ephrins in cancer: bidirectional signalling and beyond [J].
Pasquale, Elena B. .
NATURE REVIEWS CANCER, 2010, 10 (03) :165-180
[24]   Complementary Proteomic Tools for the Dissection of Apoptotic Proteolysis Events [J].
Pham, Victoria C. ;
Pitti, Robert ;
Anania, Veronica G. ;
Bakalarski, Corey E. ;
Bustos, Daisy ;
Jhunjhunwala, Suchit ;
Phung, Qui T. ;
Yu, Kebing ;
Forrest, William F. ;
Kirkpatrick, Donald S. ;
Ashkenazi, Avi ;
Lill, Jennie R. .
JOURNAL OF PROTEOME RESEARCH, 2012, 11 (05) :2947-2954
[25]   The nascent polypeptide-associated complex modulates interactions between the signal recognition particle and the ribosome [J].
Powers, T ;
Walter, P .
CURRENT BIOLOGY, 1996, 6 (03) :331-338
[26]   Proteomic analysis of the transitional endoplasmic reticulum in hepatocellular carcinoma: An organelle perspective on cancer [J].
Roy, Line ;
LaBoissiere, Sylvie ;
Abdou, Eman ;
Thibault, Genevieve ;
Hamel, Nathalie ;
Taheri, Maryam ;
Boismenu, Daniel ;
Lanoix, Joel ;
Kearney, Robert E. ;
Paiement, Jacques .
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEINS AND PROTEOMICS, 2010, 1804 (09) :1869-1881
[27]   Quantitative Analysis of BTF3, HINT1, NDRG1 and ODC1 Protein Over-Expression in Human Prostate Cancer Tissue [J].
Symes, Andrew J. ;
Eilertsen, Marte ;
Millar, Michael ;
Nariculam, Joseph ;
Freeman, Alex ;
Notara, Maria ;
Feneley, Mark R. ;
Patel, Hitenedra R. H. ;
Masters, John R. W. ;
Ahmed, Aamir .
PLOS ONE, 2013, 8 (12)
[28]   Human Beta Casein Fragment (54-59) Modulates M. bovis BCG Survival and Basic Transcription Factor 3 (BTF3) Expression in THP-1 Cell Line [J].
Thakur, Dharamsheela ;
Saxena, Reshu ;
Singh, Vandana ;
Haq, Wahajul ;
Katti, S. B. ;
Singh, Bhupendra Narain ;
Tripathi, Raj Kamal .
PLOS ONE, 2012, 7 (09)
[29]   Predominant identification of RNA-binding proteins in Fas-induced apoptosis by proteome analysis [J].
Thiede, B ;
Dimmler, C ;
Siejak, F ;
Rudel, T .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (28) :26044-26050
[30]   Towards a knowledge-based Human Protein Atlas [J].
Uhlen, Mathias ;
Oksvold, Per ;
Fagerberg, Linn ;
Lundberg, Emma ;
Jonasson, Kalle ;
Forsberg, Mattias ;
Zwahlen, Martin ;
Kampf, Caroline ;
Wester, Kenneth ;
Hober, Sophia ;
Wernerus, Henrik ;
Bjorling, Lisa ;
Ponten, Fredrik .
NATURE BIOTECHNOLOGY, 2010, 28 (12) :1248-1250