Expression and clinical significance of basic transcription factor 3 in nasopharyngeal carcinoma

被引：5
作者：
Chen, Ping [1 ]
Zhong, Qi [1 ]
Li, Zufei [1 ]
Zhang, Yang [1 ]
Huang, Zhigang [1 ]
机构：
[1] Capital Med Univ, Beijing Tongren Hosp, Dept Otolaryngol Head & Neck Surg, 1 Dongjiaomin Alley, Beijing 100730, Peoples R China
来源：
ONCOLOGY LETTERS | 2019年 / 17卷 / 01期
基金：
中国国家自然科学基金;
关键词：
basic transcription factor 3; head and neck squamous cell carcinomas; small interfering RNA; cell function; POLYPEPTIDE-ASSOCIATED COMPLEX; GENE-EXPRESSION; BETA-NAC; PROTEIN; RNA; APOPTOSIS; IDENTIFICATION; CHAPERONES;
D O I：
10.3892/ol.2018.9699
中图分类号：
R73 [肿瘤学];
学科分类号：
100214 ;
摘要：
Basic transcription factor 3 (BTF3), a transcription factor and modulator of apoptosis, is differentially expressed in carcinoma. To acquire further understanding of the involvement of BTF3 in carcinoma, the present study analyzed the expression of BTF3, as well as its role in cell function in nasopharyngeal carcinoma (NPC). BTF3 transcription rates in human NPC samples (n=46) and adjacent normal tissue samples (n=46) were analyzed using reverse transcription-quantitative polymerase chain reaction and immunohistochemistry. BTF3-silencing in NPC cells was performed via specific small interfering RNA molecules. The function of BTF3 was analyzed by proliferation assays and colony forming assays using a Cellomic assay system. The positive expression rates of BTF3 were significantly increased in cancerous tissues compared with those in adjacent tissues (P<0.05). In addition, BTF3-silencing decreased cell proliferation and colony formation (P<0.01) in TCA-8113 and 5-8F cells. BTF3 is overexpressed in NPC, and its silencing is associated with decreased cell proliferation and colony formation, enhanced apoptosis and cell cycle regulation of TCA-8113 and 5-8F cells.
引用
收藏
页码：789 / 796
页数：8
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