Croceicoccus sediminis sp. nov., isolated from coastal sediment

A Gram-stain-negative, cocci or short rod-shaped, yellow-pigmented bacterium, designated strain S2-4-2(T), was isolated from coastal sediment of Spartina alterniflora in Quanzhou Bay, PR China. Growth was observed at 15-40 degrees C (optimum, 30 degrees C) and pH 6.0-8.0 (optimum, pH 7.0). Strain S2-4-2(T) tolerated 0-10% NaCl (optimum, 1 %). The 165 rRNA gene of strain S2-4-2(T) showed highest sequence similarity to Croceicoccus pelagius Ery9(T) (98.2 %), followed by Croceicoccus naphthovorans PQ-2(T), Croceicoccus marinus E4A9(T) and Croceicoccus mobilis Ery22T (97.6%. 96.3 and 96.3%, respectively). Phylogenetic analysis based on 16S rRNA gene sequences and phylogenomic analysis based on 92 up-to-date bacterial core gene sets indicated that strain S2-4-2(T) forms a distinct monophyletic branch affiliated to the genus Croceicoccus. The average nucleotide identity value between strain S2-4-2(T) and its close relatives were estimated to be 74.8-85.7%. The respiratory quinone was found to be 0-10. The predominant fatty acids (>10%) were identified as summed feature 8 (C-18.1 omega 7c and/or C-18.1 omega 6c) and summed feature 3 (C-16.1 omega 7c and C-18.1 omega 6c). The polar lipids were identified as phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and an unidentified phospholipid. The draft genome size of strain 52-4-2(T) was 3.5 Mb with a genomic G+C content of 63.0 mol%. Based on these results, strain S2-4-2(T) is concluded to represent a novel species within the genus Croceicoccus, for which the name Croceicoccus sediminis sp. nov. is proposed with the type strain S2-4-2(T) (=MCCC 1 K03706(T)=KCTC 72146(T)).