Antimicrobial Resistance Genes and Diversity of Clones among Faecal ESBL-Producing Escherichia coli Isolated from Healthy and Sick Dogs Living in Portugal

被引：29

作者：

Carvalho, Isabel ^{[1
,2
,3
,4
,5
]}

Cunha, Rita ^{[6
]}

Martins, Carla ^{[7
]}

Martinez-Alvarez, Sandra ^{[5
]}

Safia Chenouf, Nadia ^{[5
,8
]}

Pimenta, Paulo ^{[9
]}

Pereira, Ana Raquel ^{[10
]}

Ramos, Sonia ^{[11
]}

Sadi, Madjid ^{[5
,12
]}

Martins, Angela ^{[13
]}

Facanha, Jorge ^{[14
]}

Rabbi, Fazle ^{[15
]}

Capita, Rosa ^{[16
,17
]}

Alonso-Calleja, Carlos ^{[16
,17
]}

Nunes Enes Dapkevicius, Maria de Lurdes ^{[18
,19
]}

Igrejas, Gilberto ^{[2
,3
,4
]}

Torres, Carmen ^{[5
]}

Poeta, Patricia ^{[1
,4
]}

机构：

[1] Univ Tras Os Montes & Alto Douro, Dept Vet Sci, Microbiol & Antibiot Resistance Team MicroART, P-5000801 Vila Real, Portugal

[2] Univ Tras Os Montes & Alto Douro, Dept Genet & Biotechnol, P-5000801 Vila Real, Portugal

[3] Univ Tras Os Montes & Alto Douro, Funct Genom & Prote Unit, P-5000801 Vila Real, Portugal

[4] Univ Nova Lisboa, Lab Associated Green Chem LAQV REQUIMTE, P-2829516 Monte De Caparica, Portugal

[5] Univ La Rioja, Area Biochem & Mol Biol, Logrono 26006, Spain

[6] Hosp Vet Cascais Onevet, P-2775352 Lisbon, Portugal

[7] Clin Vet Vouga, P-3740253 Sever Do Vouga, Portugal

[8] Univ Djelfa, Lab Explorat & Valuat Steppe Ecosyst, Djelfa 17000, Algeria

[9] Hosp Vet Tras Os Montes, P-5000056 Vila Real, Portugal

[10] Ctr Vet Macedo Cavaleiros, P-5340202 Braganca, Portugal

[11] Consultorio Vet Monte Redondo Unipessoal Lda, VetRedondo, P-2425618 Monte Redondo, Leiria, Portugal

[12] Univ Saad Dahlab Blida, Lab Biotechnol Related Anim Reprod, Blida 09000, Algeria

[13] Univ Tras Os Montes & Alto Douro, Anim & Vet Res Ctr CECAV, P-5000801 Vila Real, Portugal

[14] Ctr Vet Jorge Facanha, P-5140060 Carrazeda De Ansiaes, Portugal

[15] Australian Comp Soc, Melbourne, Vic 3008, Australia

[16] Univ Leon, Vet Fac, Dept Food Hyg & Technol, Leon 24071, Spain

[17] Univ Leon, Inst Food Sci & Technol, Leon 24071, Spain

[18] Univ Azores, Fac Agr & Environm Sci, P-9500321 Angra Do Heroismo, Portugal

[19] Univ Azores, Inst Agr & Environm Res & Technol IITAA, P-9500321 Angra Do Heroismo, Portugal

来源：

ANTIBIOTICS-BASEL | 2021年 / 10卷 / 08期

关键词：

antimicrobial resistance; dogs; Escherichia coli; ESBL; CTX-M-15; CTX-M-1; CTX-M-32; CTX-M-55; CTX-M-14; qAmpC; CMY-2; EXTENDED-SPECTRUM; BETA-LACTAMASES; MOLECULAR CHARACTERIZATION; HIGH PREVALENCE; MULTIPLEX PCR; PETS; BLA(CTX-M); EMERGENCE; CATS; MECHANISMS;

D O I：

10.3390/antibiotics10081013

中图分类号：

R51 [传染病];

学科分类号：

100401 ;

摘要：

The purpose of this study was to analyse the prevalence and genetic characteristics of ESBL and acquired-AmpC (qAmpC)-producing Escherichia coli isolates from healthy and sick dogs in Portugal. Three hundred and sixty-one faecal samples from sick and healthy dogs were seeded on MacConkey agar supplemented with cefotaxime (2 mu g/mL) for cefotaxime-resistant (CTX(R)) E. coli recovery. Antimicrobial susceptibility testing for 15 antibiotics was performed and the ESBL-phenotype of the E. coli isolates was screened. Detection of antimicrobial resistance and virulence genes, and molecular typing of the isolates (phylogroups, multilocus-sequence-typing, and specific-ST131) were performed by PCR (and sequencing when required). CTX(R) E. coli isolates were obtained in 51/361 faecal samples analysed (14.1%), originating from 36/234 sick dogs and 15/127 healthy dogs. Forty-seven ESBL-producing E. coli isolates were recovered from 32 sick (13.7%) and 15 healthy animals (11.8%). Different variants of bla(CTX-M) genes were detected among 45/47 ESBL-producers: bla(CTX-M-15) (n = 26), bla(CTX-M-1) (n = 10), bla(CTX-M-32) (n = 3), bla(CTX-M-55) (n = 3), bla(CTX-M-14) (n = 2), and bla(CTX-M)-variant (n = 1); one ESBL-positive isolate co-produced CTX-M-15 and CMY-2 enzymes. Moreover, two additional CTX(R) ESBL-negative E. coli isolates were CMY-2-producers (qAmpC). Ten different sequence types were identified (ST/phylogenetic-group/beta-lactamase): ST131/B2/CTX-M-15, ST617/A/CTX-M-55, ST3078/B1/CTX-M-32, ST542/A/CTX-M-14, ST57/D/CTX-M-1, ST12/B2/CTX-M-15, ST6448/B1/CTX-M-15 + CMY-2, ST5766/A/CTX-M-32, ST115/D/CMY-2 and a new-ST/D/CMY-2. Five variants of CTX-M enzymes (CTX-M-15 and CTX-M-1 predominant) and eight different clonal complexes were detected from canine ESBL-producing E. coli isolates. Although at a lower rate, CMY-2 beta-lactamase was also found. Dogs remain frequent carriers of ESBL and/or qAmpC-producing E. coli with a potential zoonotic role.

引用

页数：15

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