Analysis of 28 hair samples from users of the hallucinogenic beverage ayahuasca

被引：12

作者：

Liu, Mengxi ^{[1
,2
]}

Yang, Huan ^{[1
,2
]}

Hu, Jing ^{[2
]}

Shen, Baohua ^{[2
]}

Xiang, Ping ^{[2
]}

Qiang, Huosheng ^{[2
]}

Deng, Hongxiao ^{[2
]}

Yu, Zhiguo ^{[1
]}

Shi, Yan ^{[2
]}

机构：

[1] Shenyang Pharmaceut Univ, Sch Pharm, Wenhua Rd 103, Shenyang 110016, Peoples R China

[2] Minist Justice, Dept Forens Toxicol, Shanghai Key Lab Forens Med, Acad Forens Sci, 1347 Guangfu Xi Rd, Shanghai 200063, Peoples R China

来源：

FORENSIC SCIENCE INTERNATIONAL | 2021年 / 323卷

基金：

中国国家自然科学基金;

关键词：

Ayahuasca; Dimethyltryptamine; Hair analysis; LC-MS/MS; LIQUID-CHROMATOGRAPHY; DIMETHYLTRYPTAMINE DMT; MASS-SPECTROMETRY; ALKALOIDS; DRUG; N; N-DIMETHYLTRYPTAMINE; AMPHETAMINE; GUIDELINES; METABOLISM;

D O I：

10.1016/j.forsciint.2021.110790

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

Ayahuasca is a psychoactive beverage widely used in religious ceremonies in Amazonia. Dimethyltryptamine is the main active compound of ayahuasca. Dimethyltryptamine has many hazardous effects, including hallucinations. In the present study, a fast and reliable UPLC-MS/MS method was devel-oped and validated for the quantitation of dimethyltryptamine in hair samples. Twenty-milligram hair samples were pulverized with methanol below 4 degrees C. After ultrasonication, centrifugation and filtration, 200 mu L of supernatant was placed into an autosampler vial for LC-MS/MS analysis. The lower limit of quantitation (LLOQ) was 3 pg/mg. The resulting calibration curve for dimethyltryptamine fit the expression y = 281.50213x + 0.00231 (R-2 = 0.992). Acceptable intraday and interday precision (RSD < 15%) and accuracy (92-113%) were achieved. The dilution integrity was deemed acceptable based on accuracy (96%) and precision (1.8%). The validated method was successfully applied to 28 forensic cases. The concentrations of dimethyltryptamine ranged from 3 to 1109 pg/mg. (C) 2021 Elsevier B.V. All rights reserved.

引用

页数：6

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