Gold Nanostars with Reduced Fouling Facilitate Small Molecule Detection in the Presence of Protein

被引：21
作者：
Tukova, Anastasiia [1 ]
Kuschnerus, Inga Christine [2 ,3 ]
Garcia-Bennett, Alfonso [1 ]
Wang, Yuling [1 ]
Rodger, Alison [1 ]
机构：
[1] Macquarie Univ, Fac Sci & Engn, Dept Mol Sci, Sydney, NSW 2019, Australia
[2] Univ New South Wales, Electron Microscopy Unit, Sydney, NSW 2052, Australia
[3] Univ New South Wales, Sch Mat Sci & Engn, Sydney, NSW 2052, Australia
来源：
NANOMATERIALS | 2021年 / 11卷 / 10期
关键词：
gold nanoparticles; nanostars; nanospheroids; protein corona; surface-enhanced Raman spectroscopy; circular dichroism; cryo-TEM; SURFACE-PLASMON RESONANCE; CIRCULAR-DICHROISM; NANOPARTICLES; CORONA; NANOSTRUCTURES; NANORODS; THERAPY; SIZE;
D O I：
10.3390/nano11102565
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
Gold nanoparticles have the potential to be used in biomedical applications from diagnostics to drug delivery. However, interactions of gold nanoparticles with different biomolecules in the cellular environment result in the formation of a "protein corona "-a layer of protein formed around a nanoparticle, which induces changes in the properties of nanoparticles. In this work we developed methods to reproducibly synthesize spheroidal and star-shaped gold nanoparticles, and carried out a physico-chemical characterization of synthesized anionic gold nanospheroids and gold nanostars through transmission electron microscopy (TEM), dynamic light scattering (DLS), zeta potential (ZP), nanoparticles tracking analysis (NTA), ultraviolet-visible (UV-Vis) spectroscopy and estimates of surface-enhanced Raman spectroscopy (SERS) signal enhancement ability. We analyzed how they interact with proteins after pre-incubation with bovine serum albumin (BSA) via UV-Vis, DLS, ZP, NTA, SERS, cryogenic TEM (cryo-TEM) and circular dichroism (CD) spectroscopy. The tests demonstrated that the protein adsorption on the particles' surfaces was different for spheroidal and star shaped particles. In our experiments, star shaped particles limited the protein corona formation at SERS "hot spots ". This benefits the small-molecule sensing of nanostars in biological media. This work adds more understanding about protein corona formation on gold nanoparticles of different shapes in biological media, and therefore guides design of particles for studies in vitro and in vivo.
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页数：17
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共 60 条
[1]   Light Concentration at the Nanometer Scale [J].
Alvarez-Puebla, Ramon ;
Liz-Marzan, Luis M. ;
Javier Garcia de Abajo, F. .
JOURNAL OF PHYSICAL CHEMISTRY LETTERS, 2010, 1 (16) :2428-2434
[2]   Terminalia bellirica fruit extract-mediated synthesis of gold nanoparticles (AuNPs) and studies on antimicrobial and antioxidant activity [J].
Annavaram, Viswadevarayalu ;
Posa, Venkata Ramana ;
Lakshmi, D. Vijaya ;
Sumalatha, J. ;
Somala, Adinarayana Reddy .
INORGANIC AND NANO-METAL CHEMISTRY, 2017, 47 (05) :681-687
[3]   Thermal Enhancement with Optically Activated Gold Nanoshells Sensitizes Breast Cancer Stem Cells to Radiation Therapy [J].
Atkinson, Rachel L. ;
Zhang, Mei ;
Diagaradjane, Parmeswaran ;
Peddibhotla, Sirisha ;
Contreras, Alejandro ;
Hilsenbeck, Susan G. ;
Woodward, Wendy A. ;
Krishnan, Sunil ;
Chang, Jenny C. ;
Rosen, Jeffrey M. .
SCIENCE TRANSLATIONAL MEDICINE, 2010, 2 (55)
[4]   Gold nanoparticles in the clinical laboratory: principles of preparation and applications [J].
Azzazy, Hassan M. E. ;
Mansour, Mai M. H. ;
Samir, Tamer M. ;
Franco, Ricardo .
CLINICAL CHEMISTRY AND LABORATORY MEDICINE, 2012, 50 (02) :193-209
[5]   The Basic Properties of Gold Nanoparticles and their Applications in Tumor Diagnosis and Treatment [J].
Bai, Xue ;
Wang, Yueying ;
Song, Zhiyun ;
Feng, Yanmin ;
Chen, Yuanyuan ;
Zhang, Deyuan ;
Feng, Lin .
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2020, 21 (07)
[6]   In vivo evaluation of safety, biodistribution and pharmacokinetics of laser-synthesized gold nanoparticles [J].
Bailly, Anne-Laure ;
Correard, Florian ;
Popov, Anton ;
Tselikov, Gleb ;
Chaspoul, Florence ;
Appay, Romain ;
Al-Kattan, Ahmed ;
Kabashin, Andrei, V ;
Braguer, Diane ;
Esteve, Marie-Anne .
SCIENTIFIC REPORTS, 2019, 9 (1)
[7]   Tuning Size and Sensing Properties in Colloidal Gold Nanostars [J].
Barbosa, Silvia ;
Agrawal, Amit ;
Rodriguez-Lorenzo, Laura ;
Pastoriza-Santos, Isabel ;
Alvarez-Puebla, Raman A. ;
Kornowski, Andreas ;
Weller, Horst ;
Liz-Marzan, Luis M. .
LANGMUIR, 2010, 26 (18) :14943-14950
[8]   The Effect of Nanoparticles on Amyloid Aggregation Depends on the Protein Stability and Intrinsic Aggregation Rate [J].
Cabaleiro-Lago, C. ;
Szczepankiewicz, O. ;
Linse, S. .
LANGMUIR, 2012, 28 (03) :1852-1857
[9]   The Crown and the Scepter: Roles of the Protein Corona in Nanomedicine [J].
Cai, Rong ;
Chen, Chunying .
ADVANCED MATERIALS, 2019, 31 (45)
[10]   Time Evolution of the Nanoparticle Protein Corona [J].
Casals, Eudald ;
Pfaller, Tobias ;
Duschl, Albert ;
Oostingh, Gertie Janneke ;
Puntes, Victor .
ACS NANO, 2010, 4 (07) :3623-3632
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