Antimicrobial activity of various cationic molecules on foodborne pathogens

被引：32

作者：

Conte, Mariachiara

Aliberti, Francesco

Fucci, Laura

Piscopo, Marina

机构：

[1] Univ Naples Federico 2, Dept Struct & Funct Biol, I-80126 Naples, Italy

[2] Univ Naples Federico 2, Dept Biol Sci, I-80134 Naples, Italy

来源：

WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY | 2007年 / 23卷 / 12期

关键词：

antimicrobial activity; poly-L-arginine; cationic molecules; foodborne pathogens; protamine; POLY-L-LYSINE; PEPTIDES; BACTERIA; SYSTEM; CELLS;

D O I：

10.1007/s11274-007-9415-6

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Antibacterial effects of various arginine- and lysine-rich polycationic proteins and polymers were evaluated by broth and solid dilution assay on a range of foodborne pathogens, Gram-positive and Gram-negative bacteria. The Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of alpha-poly-L-lysine (poly-lys), alpha-poly-L-arginine (poly-arg) and protamines from herring sperm (clupeine sulphate) and salmon sperm (salmine sulphate) were determined on Bacillus subtilis, Bacillus cereus, Staphylococcus aureus, Listeria monocytogenes, Salmonella typhimurium, Shigella sonnei, Escherichia coli O157:H7 and Pseudomonas aeruginosa. All these molecules showed antibacterial activity on all strains with different MIC and MBC values. The molecular mechanisms underlying the effect of alpha-poly-L-arginine might be related to the entrance of the molecule into the cell. In fact alpha-poly-L-arginine labelled with 7-Diethylamino coumarin-3-carboxylic acid, succinimidyl ester (DEAC,SE) showed ability to permeate the cell membrane of B. cereus and E. coli O157:H7.

引用

页码：1679 / 1683

页数：5

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