Global DNA methylation in gonads of adult zebrafish Danio rerio under bisphenol A exposure

被引：60

作者：

Liu, Yan ^{[1
]}

Zhang, Yingying ^{[1
]}

Tao, Shiyu ^{[1
]}

Guan, Yongjing ^{[1
]}

Zhang, Ting ^{[1
]}

Wang, Zaizhao ^{[1
]}

机构：

[1] Northwest A&F Univ, Coll Anim Sci & Technol, Shaanxi Key Lab Mol Biol Agr, Yangling 712100, Shaanxi, Peoples R China

来源：

ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY | 2016年 / 130卷

基金：

中国国家自然科学基金;

关键词：

Bisphenol A; Gonads; Zebrafish; DNA methylation; Gene expression; MINNOW GOBIOCYPRIS-RARUS; EARLY-LIFE-STAGE; S-ADENOSYLMETHIONINE; METHYLTRANSFERASES DNMT3A; MESSENGER-RNA; GENE; EXPRESSION; LIVER; CANCER; 5-HYDROXYMETHYLCYTOSINE;

D O I：

10.1016/j.ecoenv.2016.04.012

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Altered DNA methylation is pervasively associated with changes in gene expression and signal transduction after exposure to a wide range of endocrine disrupting chemicals. As a weak estrogenic chemical, bisphenol A (BPA) has been extensively studied for reproductive toxicity. In order to explore the effects of BPA on epigenetic modification in gonads of zebrafish Danio rerio, we measured the global DNA methylation together with the gene expression of DNA methyltransferase (dnmts), glycine N-methyl-transferase (gnmt), and ten-eleven translocation (tets) in gonads of D. rerio under BPA exposure by ELISA and quantitative real-time PCR method, respectively. The global level of DNA methylation was significantly decreased in ovaries after exposed to BPA for 7 days, and testes following 35-day exposure. Moreover, the global level of DNA methylation was also significantly reduced in testes after exposed to 15 mu g/L BPA for 7 days. Besides the alteration of the global level of DNA methylation, varying degrees of transcriptional changes of dnmts, gnmt and tets were detected in gonads of D. rerio under BPA exposure. The present study suggested that BPA might cause the global DNA demethylation in gonads of zebrafish by regulating the transcriptional changes of the DNA methylation/demethylation-associated genes (dnmts, gnmt, and tets). (C) 2016 Published by Elsevier Inc.

引用

页码：124 / 132

页数：9

共 67 条

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