Cellular correlates of spontaneous periodic events in the medial entorhinal cortex of the in vitro isolated guinea pig brain

Periodic potentials characterized by fast oscillations superimposed on a slow complex event are typically observed in cortical structures during sleep and anaesthesia. In the entorhinal cortex (EC) similar spontaneus periodic events (SPEs) have been described both in vivo and in vitro. Simultaneous extracellular and intracelluar recordings from superficial neurons of the entorhinal cortex of the isolated Hartley guinea pig brain preparation demonstrated that SPEs recur with a periodicity of 2-10 s and correlate to neuronal firing superimposed on a depolarizing plateau that lasts 0.7-1 s. During SPEs, putative interneurons in all layers discharged high frequency firing (> 100 Hz), whereas no activity was observed in principal neurons of deep entorhinal cortex layers. Linear correlation analysis demonstrated a tight relationship between the fast component of the extracellular SPE and subthreshold oscillatory activity/neuronal firing in both superficial neurons and putative interneurons; firing of deep principal cells was independent from SPEs occurrence. The present study demonstrates that recurrent spontaneous events analogous to periodic activity observed during sleep/anaesthesia are generated in the entorhinal cortex by the interactions between superficial neurons and interneurons.