Nuclear export of cutaneous HPV8 E7 oncoprotein is mediated by a leucine-rich nuclear export signal via a CRM1 pathway

被引：2

作者：

Onder, Zeynep ^{[1
]}

Chang, Vivian ^{[1
]}

Moroianu, Junona ^{[1
]}

机构：

[1] Boston Coll, Dept Biol, Chestnut Hill, MA 02467 USA

来源：

VIROLOGY | 2015年 / 474卷

基金：

美国国家卫生研究院;

关键词：

Cutaneous HPV8 E7 oncoprotein; Nuclear export; CRM1; ZINC-BINDING DOMAIN; HUMAN-PAPILLOMAVIRUS TYPE-16; HYDROPHOBIC INTERACTIONS; SKIN-CANCER; CELL-CYCLE; PROTEIN; IMPORT; KERATINOCYTES; LOCALIZATION; GENES;

D O I：

10.1016/j.virol.2014.10.012

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We recently determined that the nuclear import of cutaneous beta genus HPV8 E7 oncoprotein it is mediated by its zinc-binding domain via direct hydrophobic interactions with the FG nucleoporins Nup62 and Nup153 (Onder and Moroianu, 2014). Here we investigated the nuclear export of HPV8 E7 oncoprotein using confocal microscopy after transfections of HeLa cells with EGFP-8cE7 and mutant plasmids and treatment with Ratjadone A nuclear export inhibitor. We determined that HPV8 E7 contains a leucine-rich nuclear export signal (NES), (76)IRTFQELLF(84), within its zinc-binding domain that mediates its nuclear export via a CRM1 pathway. We found that HPV8 E7 interacts with CRM1 and that the hydrophobic amino acid residues I76, F79 and L82 of the NES are essential for this interaction and for nuclear export of HPV8 E7 oncoprotein. (C) 2014 Elsevier Inc. All rights reserved.

引用

页码：28 / 33

页数：6