Three-Dimensional Reconstruction Methods for Caenorhabditis elegans Ultrastructure

被引：30

作者：

Mueller-Reichert, Thomas ^{[1
]}

Mancuso, Joel ^{[2
]}

Lich, Ben ^{[3
]}

McDonald, Kent ^{[4
]}

机构：

[1] Tech Univ Dresden, Med Theoret Ctr, D-01307 Dresden, Germany

[2] Gatan Inc, Pleasanton, CA 94588 USA

[3] FEI Co, NL-5651 Eindhoven, Netherlands

[4] Univ Calif Berkeley, Electron Microscope Lab, Berkeley, CA 94720 USA

来源：

ELECTRON MICROSCOPY OF MODEL SYSTEMS | 2010年 / 96卷

关键词：

ELECTRON-MICROSCOPY; CORRELATIVE LIGHT; CRYOELECTRON MICROSCOPY; VITREOUS SECTIONS; CUTICLIN EPITOPES; NERVOUS-SYSTEM; NEMATODE; TOMOGRAPHY; MICROTUBULES; CELLS;

D O I：

10.1016/S0091-679X(10)96015-9

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

The roundworm Caenorhabditis elegans is one of the major model organisms in modern cell and developmental biology. Here, we present methods for the three-dimensional (3D) reconstruction of the worm ultrastructure. We describe the use of (1) serial-section analysis, (2) electron tomography, and (3) serial block face imaging by scanning electron microscopy (SEM). Sample preparation for high-pressure freezing/freeze substitution (HPF/FS) has been extensively covered in a previous volume of this "Methods in Cell Biology" series and will only be described briefly. We will discuss these 3D methods in light of recent research activities related to worm and early embryo biology.

引用

页码：331 / 361

页数：31

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