Simultaneous determination of arsenic and selenium in biological samples by HG-AFS

被引：29

作者：

Sun, HW ^{[1
]}

Liu, ZF

Wu, WJ

Li, LQ

Shi, HM

机构：

[1] Hebei Univ, Coll Chem & Environm Sci, Key Lab Analyt Sci & Technol Hebei Prov, Baoding 071002, Peoples R China

[2] Hebei Med Univ, Hosp 3, Dept Radiol, Shijiazhuang 050051, Peoples R China

[3] Taishan Coll, Dept Chem, Shan Dong Taian 271021, Peoples R China

来源：

ANALYTICAL AND BIOANALYTICAL CHEMISTRY | 2005年 / 382卷 / 04期

关键词：

simultaneous determination; arsenic; selenium; biological samples; atomic fluorescence spectrometry;

D O I：

10.1007/s00216-005-3226-0

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A new method is proposed for simultaneous determination of traces of arsenic (As) and selenium (Se) in biological samples by hydride-generation double-channel non-dispersive atomic-fluorescence spectrometry (HG-AFS) from tartaric acid media. The effects of analytical conditions on fluorescence signal intensity were investigated and optimized. Interferences from coexisting ions were evaluated. Under optimum conditions linear response ranges above 20 mu g L-1 for As and 32 mu g L-1 for Se were obtained with detection limits of 0.13 and 0.12 mu g L-1, respectively. The precision for elevenfold determination of As at the 4 mu g L-1 level and of Se at the 8 mu g L-1 level were 2.7 and 1.9% (RSD), respectively. Recoveries of 92.5-95.5% for As and 101.2-108.4% for Se were obtained for four biological samples and two certified biological reference materials. The proposed method has the advantages of simple operation, high sensitivity, and high efficiency; it was successfully used for simultaneous determination of As and Se in biological samples.

引用

页码：1060 / 1065

页数：6

共 19 条

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